Okello Pius, Bjöersdorff Olivia Graaf, Hansson Ingrid, Boqvist Sofia, Erume Joseph
College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University Kampala, Kampala, Uganda.
Department of Animal Biosciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
PLoS One. 2025 Jan 30;20(1):e0318516. doi: 10.1371/journal.pone.0318516. eCollection 2025.
Campylobacteriosis is one of the most commonly reported foodborne diseases and is of particular importance in low-income countries. More data is needed to better understand the epidemiology of Campylobacter spp. in food sold at informal markets, where most people in low-income countries buy their food. This study aimed to determine the prevalence and antimicrobial resistance (AMR) of Campylobacter spp. among broiler chicken carcasses sold at informal urban markets in Uganda and to gain more knowledge about hygienic handling practices and awareness of foodborne bacterial diseases among the market vendors. In total, 120 broiler chicken carcasses from 30 different markets were analysed using ISO 10272 and confirmed by PCR. AMR analyses were performed using the disc diffusion test. Epidemiological data on food safety practices and awareness was collected from the vendors using a questionnaire. Campylobacter spp. was isolated from 66% (79/120) of the carcasses; 32% were C. jejuni, 14% were C. coli and 54% comprised of a mixture of both species. All C. jejuni isolates showed resistance to tetracycline, 88% to ciprofloxacin and 28% to erythromycin. Of the C. coli isolates, 82% showed resistance to tetracycline, 73% to erythromycin and the quinolones ciprofloxacin and nalidixic acid. More than half of the vendors had heard about food-borne illnesses, but none knew about Campylobacter spp., and the knowledge regarding hygienic practices was low. These data calls for urgent interventions to improve food safety, protect the public from foodborne illness, and prevent the spreading of AMR.
弯曲杆菌病是最常报告的食源性疾病之一，在低收入国家尤为重要。需要更多数据来更好地了解在非正规市场销售的食品中弯曲杆菌属的流行病学情况，低收入国家的大多数人都在这些市场购买食品。本研究旨在确定乌干达城市非正规市场销售的肉鸡胴体中弯曲杆菌属的流行情况和抗菌药物耐药性（AMR），并更多地了解市场摊贩的卫生处理做法和对食源性细菌疾病的认识。总共使用ISO 10272对来自30个不同市场的120个肉鸡胴体进行了分析，并通过PCR进行了确认。使用纸片扩散法进行AMR分析。使用问卷从摊贩那里收集了关于食品安全做法和认识的流行病学数据。弯曲杆菌属从66%（79/120）的胴体中分离出来；32%为空肠弯曲菌，14%为结肠弯曲菌，54%为两种菌种的混合物。所有空肠弯曲菌分离株均对四环素耐药，88%对环丙沙星耐药，28%对红霉素耐药。在结肠弯曲菌分离株中，82%对四环素耐药，73%对红霉素以及喹诺酮类药物环丙沙星和萘啶酸耐药。超过一半的摊贩听说过食源性疾病，但没有人了解弯曲杆菌属，并且关于卫生做法的知识水平较低。这些数据呼吁采取紧急干预措施，以改善食品安全，保护公众免受食源性疾病的侵害，并防止AMR的传播。
