Pirouzzadeh Maryam, Moraffah Fatemeh, Samadi Nasrin, Sharifzadeh Mohammad, Motasadizadeh Hamidreza, Vatanara Alireza
Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1414614411, Iran.
Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran 1414614411, Iran.
Int J Biol Macromol. 2025 Apr;301:140454. doi: 10.1016/j.ijbiomac.2025.140454. Epub 2025 Jan 28.
Wound infection can prolong the healing process, leading to various complications. Although the use of antibiotics is common, it presents challenges such as poor pharmacokinetics. The prevalence of antibiotic resistance has further complicated wound management. Studies have demonstrated the potential of topical probiotics; however, stabilizing these cells and creating appropriate conditions for their colonization are essential. In this study, polymeric films were utilized to immobilize probiotics and provide an effective dressing. Aloe vera gel was incorporated into the films to enhance anti-inflammatory activity and probiotic viability. The optimal film was developed using sodium alginate and zinc chloride, with manufacturing parameters optimized to achieve the highest cell viability and suitable properties through the D-optimal method. The models were verified by comparing the actual and predictive responses of the optimized formulation. The optimal film was characterized using microscopic, mechanical, and microbial examinations. The cross-linked alginate matrix improved the stability of probiotics during storage. In vivo tests demonstrated that the formulation could reduce microbial counts and shorten healing duration. This dressing improved wound healing with immobilized probiotics and aloe vera gel, offering promising directions for future research on advanced biomaterials to enhance wound care and combat antibiotic resistance.
伤口感染会延长愈合过程，导致各种并发症。尽管使用抗生素很常见，但它存在诸如药代动力学不佳等挑战。抗生素耐药性的普遍存在使伤口管理更加复杂。研究已经证明了局部使用益生菌的潜力；然而，稳定这些细胞并为其定植创造合适的条件至关重要。在本研究中，利用聚合物薄膜固定益生菌并提供有效的敷料。将芦荟凝胶掺入薄膜中以增强抗炎活性和益生菌活力。使用海藻酸钠和氯化锌开发了最佳薄膜，并通过D - 最优方法优化制造参数，以实现最高的细胞活力和合适的性能。通过比较优化配方的实际响应和预测响应来验证模型。使用显微镜、力学和微生物检查对最佳薄膜进行表征。交联的海藻酸盐基质提高了益生菌在储存期间的稳定性。体内试验表明，该配方可以减少微生物数量并缩短愈合持续时间。这种含有固定化益生菌和芦荟凝胶的敷料改善了伤口愈合，为未来关于先进生物材料以加强伤口护理和对抗抗生素耐药性的研究提供了有前景的方向。
