荞麦（苦荞麦）形态部位膳食纤维和多酚含量的研究

The Content of Dietary Fibre and Polyphenols in Morphological Parts of Buckwheat (Fagopyrum tataricum).

机构信息

Institute of Food Technology and Plant Origin, Poznan University of Life Sciences, Wojska Polskiego 31, Poznań, Poland.

Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Szpitalna 27/33, 60-572, Poznań, Poland.

出版信息

Plant Foods Hum Nutr. 2018 Mar;73(1):82-88. doi: 10.1007/s11130-018-0659-0.

DOI:10.1007/s11130-018-0659-0

PMID:29435700

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5829111/

Abstract

In this report, we presented the profile of polyphenolic substances in flowers, leaves, stalk and roots of Fagopyrum tataricum estimated by using RP-UHPLC-ESI-MS equipment (reversed-phase ultra-high-performance liquid chromatography electrospray ionisation mass spectrometry). The neutral detergent fibre, acid detergent fibre, acid detergent lignin, cellulose and hemicellulose were also determined. Flowers, leaves, stalk and roots showed varying levels of dietary fibre and polyphenols. The highest content of neutral and acid detergent fibre were found in the roots (63.92 and 45.45% d.m., respectively) while the most rich in phenolic compounds were flowers (4.8 mg/1 g d.m.). Root and stalk contained the highest level of cellulose, 38.70 and 25.57% d.m., respectively. Among the investigated polyphenolic substances such as: 2,6-dihydroxybenzoic acid, 3,4-dihydroxybenzoic acid, 3,5-dihydroxybenzoic acid, 4-hydrobenzoic acid, caffeic acid, catechin, chlorogenic acid, fagopyrin, ferulic acid, myricetin, gallic acid, isovanilic acid, isovitexin, kaempferol, luteolin, p-coumaric acid, procyanidin B2, quercetin, quercetin 3-D galactoside, rutin, syringic acid and vitexin, we observed that the contents of rutin and chlorogenic acid were the highest. We found some correlation between dietary fibre fractions and individual phenolic substances. The levels of acid detergent fibre (ADF), cellulose and hemicellulose were negatively correlated with isovitexin, kaempferol, vitexin, fagopyrin, caffeic acid and procyanidin B2 content. In this investigation, two solvents (water and methanol) were estimated regarding their extraction efficiency of phenolic compounds. Taking these results into consideration, we recommend using methanol as the extractor to isolate chlorogenic acid, fagopyrin, kaempferol, procyanidin B2, quercetin, quercetin 3-D-galactoside, rutin, vitexin, and water for other investigated polyphenolic substances obtained from Fagopyrum tataricum.

摘要

在本报告中，我们使用 RP-UHPLC-ESI-MS 设备（反相超高效液相色谱电喷雾电离质谱）估算了苦荞麦花、叶、茎和根中的多酚物质的特征。还测定了中性洗涤剂纤维、酸性洗涤剂纤维、酸性洗涤剂木质素、纤维素和半纤维素。花、叶、茎和根显示出不同水平的膳食纤维和多酚。根中中性和酸性洗涤剂纤维的含量最高（分别为 63.92%和 45.45%干物质），而花中酚类化合物含量最丰富（4.8mg/1g 干物质）。根和茎含有最高水平的纤维素，分别为 38.70%和 25.57%干物质。在所研究的多酚物质中，如：2,6-二羟基苯甲酸、3,4-二羟基苯甲酸、3,5-二羟基苯甲酸、4-羟基苯甲酸、咖啡酸、儿茶素、绿原酸、荞麦苷、阿魏酸、杨梅素、没食子酸、香草酸、异荭草苷、异荭草素、山柰酚、木樨草素、对香豆酸、原花青素 B2、槲皮素、槲皮素 3-D 半乳糖苷、芦丁、丁香酸和牡荆素，我们观察到芦丁和绿原酸的含量最高。我们发现膳食纤维各部分与个别酚类物质之间存在一定的相关性。酸性洗涤剂纤维（ADF）、纤维素和半纤维素的水平与异荭草苷、山柰酚、牡荆素、荞麦苷、咖啡酸和原花青素 B2 的含量呈负相关。在本研究中，我们比较了两种溶剂（水和甲醇）对酚类化合物提取效率。考虑到这些结果，我们建议使用甲醇作为提取剂来分离苦荞麦中的绿原酸、荞麦苷、山柰酚、原花青素 B2、槲皮素、槲皮素 3-D-半乳糖苷、芦丁、牡荆素和水，以获得其他研究中从苦荞麦中获得的酚类物质。