Fernandes Hocelayne Paulino, Salomé-Abarca Luis Francisco
Natural Products Laboratory, Institute of Biology, Leiden University, BE, Leiden, The Netherlands.
Laborotorio de Química de Products Naturales, Departmento de Biotecnologia y Bioquímica, Centro de Investigatión y de Estudios Avanzados de IPN, Guanajuato, Mexico.
Methods Mol Biol. 2025;2895:153-163. doi: 10.1007/978-1-0716-4350-1_11.
Coumarins are a class of natural compounds that exhibit a wide range of biological activities, which include anticoagulant, anti-inflammatory, anticancer, and antimicrobial effects. This class of compounds has drawn considerable interest in recent years. The exploration of such properties contributes to our understanding of the potential of coumarins as valuable agents in various clinical applications. Thus, several scientific articles providing a comprehensive exploration of the isolation of coumarins from various plant species have been published. Therefore, this chapter presents an already tested protocol that ensures the effective extraction and isolation of different coumarins from leaves of Citrus latifolia, achieved by liquid and planar chromatography. The identification of coumarins is performed by TLC and H NMR analysis.
香豆素是一类具有广泛生物活性的天然化合物，这些生物活性包括抗凝血、抗炎、抗癌和抗菌作用。近年来，这类化合物引起了相当大的关注。对其此类特性的探索有助于我们了解香豆素作为各种临床应用中有价值药物的潜力。因此，已经发表了几篇科学文章，全面探索了从各种植物物种中分离香豆素的方法。因此，本章介绍了一种已经经过测试的方案，该方案通过液相色谱和平面色谱法，确保从宽皮柠檬叶片中有效提取和分离不同的香豆素。香豆素的鉴定通过薄层色谱法（TLC）和氢核磁共振（H NMR）分析进行。
