Gönen Zeynep Burçin, Kemaloğlu Cemal Alper, Gökdemir Nur Seda, Soylu Emrah, Bolat Demet, Yay Arzu
Department of Oral and Maxillofacial Surgery, Genome and Stem Cell Centre, Faculty of Dentistry, Erciyes University.
Department of Plastic, Reconstructive and Aesthetic Surgery, Erciyes University, School of Medicine.
J Craniofac Surg. 2025 Jun 1;36(4):1425-1429. doi: 10.1097/SCS.0000000000011087. Epub 2025 Jan 31.
Although scaffold materials serve as effective bone substitutes for mandibular reconstruction, their high resorption and biodegradation rates decrease the success of the therapeutic effect. This study aims to explore the bone regenerative potential of bovine-derived osteoid matrix xenografts coupled with cell-free treatments. The study was conducted as a randomized in vivo experiment to repair critical-sized defects in rabbit mandibles. Two treatment groups were established with 10 rabbits: the right hemimandibles were reconstructed with xenograft (control), and the left hemimandibles were reconstructed with dental pulp stem cell (DPSCs)-derived exosomes soaked xenograft (experimental group) within the same rabbit. Micro-computed tomography and histologic analysis were performed at 4 weeks postoperatively. The histopathologic analysis revealed a significant difference in new bone formation scores between the experimental group (4.87±0.78) and the control group (2.87±0.41). Although the micro-computer tomography analysis did not show a statistically significant difference in new bone volume and surface between the groups, it did reveal that the experimental group had greater bone mineral density, trabecular thickness, and separation. The combination of DPSCs-derived exosomes and xenografts proved to be a promising strategy for enhancing new bone formation and regenerative scores in repairing critical-size defects in rabbit mandibles.
尽管支架材料可作为下颌骨重建的有效骨替代物，但其高吸收率和生物降解率会降低治疗效果的成功率。本研究旨在探索牛源类骨质基质异种移植物与无细胞处理相结合的骨再生潜力。该研究作为一项随机体内实验进行，以修复兔下颌骨的临界尺寸缺损。将10只兔子分为两个治疗组：在同一只兔子体内，右侧半下颌骨用异种移植物重建（对照组），左侧半下颌骨用牙髓干细胞（DPSC）衍生的外泌体浸泡的异种移植物重建（实验组）。术后4周进行微型计算机断层扫描和组织学分析。组织病理学分析显示，实验组（4.87±0.78）和对照组（2.87±0.41）的新骨形成评分存在显著差异。尽管微型计算机断层扫描分析未显示两组之间在新骨体积和表面方面有统计学显著差异，但确实显示实验组具有更高的骨矿物质密度、小梁厚度和间距。牙髓干细胞衍生的外泌体与异种移植物的组合被证明是一种有前景的策略，可提高兔下颌骨临界尺寸缺损修复中的新骨形成和再生评分。
