Alkaisi Amera, Ismail Abd Rashid, Mutum Samarendra S, Ahmad Zainal A Rifin, Masudi Sam'an, Abd Razak Noor Hayati
Lectural, PhD Student, Universiti Sains Malaysia; Lectural in University of Anbar, College of Dentistry; Oral and Maxillofacial Department, Alramady, Iraq.
J Oral Maxillofac Surg. 2013 Oct;71(10):1758.e1-13. doi: 10.1016/j.joms.2013.05.016.
The main aim of the present study was to evaluate the capacity of stem cells from human exfoliated deciduous teeth (SHED) to enhance mandibular distraction osteogenesis (DO) in rabbits.
A randomized controlled trial was conducted. Eighteen skeletally immature New Zealand white rabbits were divided into 2 groups, with 9 in the control group and 9 in the SHED group. The SHED were isolated, expanded, and characterized. Six million cells were transplanted into the distracted area during the osteotomy period. After a 4-day latency period, a total of 6 mm was distracted for 6 days. The newly formed bone was analyzed radiologically, histologically, and histomorphometrically at 2, 4, and 6 weeks postoperatively. Nonparametric analysis of variance (Kruskal-Wallis test) was used for data analysis, and P < .05 was considered statistically significant.
The cell lineage was positive for the 2 mesenchymal stem cell markers tested (CD105 and CD166). More mature bone in the SHED transplanted group was observed radiographically and histologically. Histomorphologically, the percentage of newly formed bone after 2, 4, and 6 weeks was 18.41% and 41.53%, 31.68% and 59.78%, and 52.34% and 65.24% in the control and SHED groups, respectively. The difference between the groups was statistically significant (P = .012). The bone union and stage of bone maturity scores were significantly different between the control and SHED groups (P = .006 and P = .011, respectively).
Our findings suggest that SHED can serve as an additional cell resource for DO enhancement in rabbits and might be a promising model for the reconstruction of large mandibular defects in human oral maxillofacial surgery.
本研究的主要目的是评估人乳牙脱落干细胞（SHED）增强兔下颌骨牵张成骨（DO）的能力。
进行了一项随机对照试验。18只骨骼未成熟的新西兰白兔分为2组，对照组9只，SHED组9只。分离、扩增并鉴定SHED。在截骨期将600万个细胞移植到牵张区域。经过4天的潜伏期后，共牵张6mm，持续6天。术后2、4和6周对新形成的骨进行放射学、组织学和组织形态计量学分析。数据分析采用非参数方差分析（Kruskal-Wallis检验），P <.05被认为具有统计学意义。
所检测的两种间充质干细胞标志物（CD105和CD166）的细胞谱系呈阳性。在放射学和组织学上观察到SHED移植组有更成熟的骨。组织形态计量学分析显示，术后2、4和6周时，对照组新形成骨的百分比分别为18.41%和41.53%、31.68%和59.78%、52.34%和65.24%，SHED组分别为上述对应值。两组间差异具有统计学意义（P = 0.012）。对照组和SHED组的骨愈合和骨成熟阶段评分有显著差异（分别为P = 0.006和P = 0.011）。
我们的研究结果表明，SHED可作为增强兔DO的额外细胞来源，可能是人类口腔颌面外科重建大型下颌骨缺损的一个有前景的模型。
