A fluorescence biosensor based on DNA aptamers-COF for highly selective detection of ATP and thrombin.

Due to their distinctive physical, chemical, electrical, and optical properties as well as their prospective uses, 2D covalent organic framework (COF) have attracted much attention. Herein, TaTPA-COF was effectively synthesized from the condensation of TTA and TFPA using a facile solvothermal method and characterized by SEM image, FT-IR spectra, and PXRD pattern. The generated bulk TaTPA-COF materials combined with DNA aptamers are utilized as the acceptor (quencher) for the highly sensitive and selective detection of adenosine 5'-triphosphate (ATP) and thrombin, with a novel fluorescence biosensing platform and a proof-of-concept application.