Xu Tongtong, Liang Yueyin, Gong Shuai, Meng Zhiyuan, Zhang Yan, Huang Shun, Liu Tianqi, Wang Shifa, Wang Zhonglong
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, College of Light Industry and Food, Nanjing Forestry University, Nanjing 210037 PR China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, College of Light Industry and Food, Nanjing Forestry University, Nanjing 210037 PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 May 5;332:125800. doi: 10.1016/j.saa.2025.125800. Epub 2025 Jan 26.
The excessive addition of hypochlorous acid (HClO) during food preservation can reduce the nutritional value of foodstuffs, and its abnormal levels in organisms will affect normal physiological and pathological activities. Herein, an efficient fluorescent probe HTTPC for monitoring HClO in foodstuffs and biosystems has been developed from natural flavonol. HTTPC itself emitted intense orange fluorescence emission in nearly 100 % aqueous solution, and it underwent a remarkable fluorescence quenching effect after being treated with HClO. HTTPC could selectively recognize HClO over other different biologically relevant species. Moreover, HTTPC featured many distinguished sensing properties, including large Stokes shift (184 nm), low detection limit (4.7 nM), strong anti-interference capability and rapid response to HClO (10 s). Furthermore, HTTPC can serve as a potential approach for qualitative determination of HClO residues within foodstuffs with good recoveries. HTTPC exhibited outstanding biocompatibility and low cytotoxicity, and it could successfully achieve the real-time fluorescence imaging of HClO fluctuations within live HeLa cells, zebrafish and onion tissues.
在食品保鲜过程中过量添加次氯酸（HClO）会降低食品的营养价值，并且其在生物体内的异常水平会影响正常的生理和病理活动。在此，一种用于监测食品和生物系统中HClO的高效荧光探针HTTPC已由天然黄酮醇开发而成。HTTPC本身在近100%的水溶液中发出强烈的橙色荧光，在用HClO处理后，它经历了显著的荧光猝灭效应。HTTPC能够比其他不同的生物相关物种更具选择性地识别HClO。此外，HTTPC具有许多突出的传感特性，包括大斯托克斯位移（184nm）、低检测限（4.7 nM）、强抗干扰能力以及对HClO的快速响应（10秒）。此外，HTTPC可作为一种潜在方法用于食品中HClO残留的定性测定，回收率良好。HTTPC表现出出色的生物相容性和低细胞毒性，并且能够成功实现活HeLa细胞、斑马鱼和洋葱组织内HClO波动的实时荧光成像。
