Liang Yueyin, Xu Tongtong, Yue Xiaoya, Xu Song, Xu Xu, Wang Shifa, Wang Zhonglong
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
J Hazard Mater. 2025 May 5;488:137425. doi: 10.1016/j.jhazmat.2025.137425. Epub 2025 Jan 27.
Hydrazine (NH) and hypochlorous acid (HClO) are significant chemicals that gain widespread applications in industrial fields. Excessive NH and HClO can cause severe environmental pollution and have toxic effects on living organisms. In this study, a near-infrared (NIR) dual-responsive fluorescent probe PTZ-BA was designed for the ratiometric detection of NH and HClO. PTZ-BA exhibited a remarkable near-infrared signal at 680 nm with a large Stokes shift of 275 nm, and it can simultaneously detect NH and HClO through different fluorescence channels without signal crosstalk. PTZ-BA possessed good selectivity, low detection limits, dramatic emission wavelength shift, short response time for the detection of NH and HClO. PTZ-BA could be employed for the quantitative analysis of NH and HClO in environmental soil and water samples, respectively. Furthermore, the functionalized nanofiber membranes and hydrogels were readily fabricated using this probe, enabling the portable on-site detection of gaseous NH and HClO with the help of smartphone. PTZ-BA could achieve the real-time tracking of NH and HClO in HeLa cells, onion cells and zebrafish. This research offers a versatile fluorescence detection platform for NH and HClO, holding a significant potential for environmental analysis and public health monitoring.
肼（NH）和次氯酸（HClO）是在工业领域有广泛应用的重要化学品。过量的NH和HClO会造成严重的环境污染，并对生物体产生毒性作用。在本研究中，设计了一种近红外（NIR）双响应荧光探针PTZ-BA用于对NH和HClO进行比率检测。PTZ-BA在680 nm处呈现出显著的近红外信号，斯托克斯位移大，为275 nm，并且它可以通过不同的荧光通道同时检测NH和HClO，而不会产生信号串扰。PTZ-BA具有良好的选择性、低检测限、显著的发射波长位移、检测NH和HClO的响应时间短。PTZ-BA可分别用于环境土壤和水样中NH和HClO的定量分析。此外，使用该探针可以轻松制备功能化纳米纤维膜和水凝胶，借助智能手机实现对气态NH和HClO的便携式现场检测。PTZ-BA能够对HeLa细胞、洋葱细胞和斑马鱼中的NH和HClO进行实时跟踪。本研究为NH和HClO提供了一个多功能荧光检测平台，在环境分析和公共卫生监测方面具有巨大潜力。
