Zhang Mengde, Hou Linhao, Song Wei, Tan Yaxin, Huang Yuyan, Li Zhao, Huang Xing, Kong Yi, Zhang Chao, Liang Liting, Feng Yu, Liu Qinghua, Fu Xiaobing, Huang Sha
Research Center for Tissue Repair and Regeneration affiliated to the Medical Innovation Research Department, PLA General Hospital, PLA Medical College, PLA Key Laboratory of Tissue Repair and Regenerative Medicine, Beijing Key Research Laboratory of Skin Injury, Repair and Regeneration, Research Unit of Trauma Care, Tissue Repair and Regeneration, Chinese Academy of Medical Sciences, 2019RU051, Beijing 100048, PR China.
Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, 29 Zhongguancun East Road, Beijing 100190, PR China.
Int J Biol Macromol. 2025 Apr;302:140549. doi: 10.1016/j.ijbiomac.2025.140549. Epub 2025 Jan 31.
Alginate, a recognized biosafe material with inherent hemostatic properties, has been widely explored for emergency hemostatic applications and wound dressings. However, advancements in crosslinking strategies and the integration of additional functionalities are essential to expand its clinical versatility. In this study, we report the development of a novel alginate-based bioink designed to address urgent clinical challenges posed by incompressible and irregular site bleeding. Our bioink, characterized by rapid photocrosslinking and sustained antibacterial effects, offers a promising solution for hemorrhage control and wound repair. The developed bioink, AL@FM, is an injectable photopolymerized alginate-based hydrogel incorporating functional modifiers (PVP, CMC, glycerol and ε-PL) that enhance its rapid hemostasis, antibacterial action, and wound healing properties, demonstrating exceptional biocompatibility in extensive in vitro and in vivo evaluations. AL@FM exhibits superior hemostatic efficiency compared to commercial controls, effectively accelerating wound contraction and angiogenesis, while displaying potent antibacterial properties and improved injectability. The integration of these multifaceted features into an alginate-based platform extends its applicability, particularly in the context of in situ 3D bioprinting where rapid gelation and immediate functionality are critical.
海藻酸盐是一种公认的具有固有止血特性的生物安全材料，已被广泛用于紧急止血应用和伤口敷料。然而，交联策略的进步以及附加功能的整合对于扩大其临床多功能性至关重要。在本研究中，我们报告了一种新型海藻酸盐基生物墨水的开发，旨在应对不可压缩和不规则部位出血带来的紧迫临床挑战。我们的生物墨水具有快速光交联和持续抗菌作用的特点，为出血控制和伤口修复提供了一个有前景的解决方案。所开发的生物墨水AL@FM是一种可注射的光聚合海藻酸盐基水凝胶，包含功能改性剂（聚乙烯吡咯烷酮、羧甲基纤维素、甘油和ε-聚赖氨酸），这些改性剂增强了其快速止血、抗菌作用和伤口愈合特性，在广泛的体外和体内评估中显示出优异的生物相容性。与商业对照相比，AL@FM表现出卓越的止血效率，有效加速伤口收缩和血管生成，同时展现出强大的抗菌性能和改善的可注射性。将这些多方面的特性整合到基于海藻酸盐的平台中扩展了其适用性，特别是在原位3D生物打印的背景下，快速凝胶化和即时功能至关重要。
