Aafria Shatrughan, Sharma Minakshi
Department of Zoology, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.
Department of Zoology, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.
Anal Biochem. 2025 May;700:115798. doi: 10.1016/j.ab.2025.115798. Epub 2025 Jan 31.
Acrylamide is a hazardous substance present in heat-processed food products and industrial wastewater. It is carcinogenic and neurotoxic and therefore emphasises the importance of monitoring its levels and the need for sensitive and accurate detection techniques. Electrochemical biosensing has emerged as a potential analytical method for detecting acrylamide. This article provides a comprehensive overview of the most recent developments in electrochemical biosensing methods, including amperometric, potentiometric, and impedimetric biosensors for acrylamide detection. The creation and use of novel biorecognition components, such as enzymes, antibodies, and molecularly imprinted polymers that enhance the sensitivity and specificity of acrylamide monitoring, are given special attention. Incorporating nanomaterials such as carbon-based nanomaterials and metallic nanoparticles was investigated for its potential to improve the sensors' electrochemical characteristics and overall efficacy. The potential of electrochemical biosensors for acrylamide detection is further illustrated, showcasing their effectiveness in a range of matrices in different food products. This review aims to inform researchers about the latest technological developments, trends, and future directions in electrochemical biosensing for acrylamide detection. The study highlights the significance of ongoing research and cooperation in creating efficient biosensing systems to protect public health and the environment by thoroughly examining current technology and pointing out areas for improvement.
丙烯酰胺是一种存在于热加工食品和工业废水中的有害物质。它具有致癌性和神经毒性，因此凸显了监测其含量水平的重要性以及对灵敏且准确的检测技术的需求。电化学生物传感已成为检测丙烯酰胺的一种潜在分析方法。本文全面概述了电化学生物传感方法的最新进展，包括用于检测丙烯酰胺的安培型、电位型和阻抗型生物传感器。特别关注了新型生物识别元件的创建和应用，例如可提高丙烯酰胺监测灵敏度和特异性的酶、抗体及分子印迹聚合物。研究了引入碳基纳米材料和金属纳米颗粒等纳米材料对改善传感器电化学特性及整体效能的潜力。进一步阐述了电化学生物传感器用于检测丙烯酰胺的潜力，展示了其在不同食品的一系列基质中的有效性。本综述旨在让研究人员了解丙烯酰胺检测电化学生物传感的最新技术进展、趋势及未来方向。该研究强调了持续研究与合作在创建高效生物传感系统以保护公众健康和环境方面的重要性，通过全面审视现有技术并指出改进方向实现这一目标。
