Rabee Mahmoud, Said Ragab A M, Alqarni Mohammed, Naguib Ibrahim A
Research and Development Department, Heliopolis University, Cairo, 11785, Egypt.
Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo, 11751, Egypt.
BMC Chem. 2025 May 20;19(1):134. doi: 10.1186/s13065-025-01501-6.
Acrylamide (ACM) is a food processing contaminant classified as a probable genotoxic and carcinogenic substance for humans. The rapid and economical determination of ACM in food products poses a major challenge for food safety. This research intended to fabricate a simple, selective, and cost-effective polymeric-coated graphite sensor. This potentiometric sensor is suitable for direct and in situ ACM analysis in food products without tedious sample pretreatment procedures. The sensor was successfully developed based on the ion association complex of the ACM cation with sodium tetraphenylborate (TPB) anion as an ion exchange site, using dibutyl phthalate (DBP) as a plasticizer. The sensor demonstrated a fast, stable, selective, and linear Nernstian response (57.45 mV/decade) over a wide concentration range from 1 × 10 to 1 × 10 M of ACM, with a detection limit of 1 × 10 M. The sensor's selectivity behavior, response time, lifetime, pH working range, and fundamental validation parameters were assessed. Compared to a published chromatographic method, the developed sensor operated effectively to determine the ACM content in several food products. Greenness and whiteness were also assessed for the developed sensor, confirming that it is an excellent green and cost-effective option. Furthermore, the developed sensor was compared statistically with recently published ACM sensors to ensure optimal performance.
丙烯酰胺（ACM）是一种食品加工污染物，被归类为对人类可能具有遗传毒性和致癌性的物质。快速且经济地测定食品中的ACM对食品安全构成了重大挑战。本研究旨在制造一种简单、选择性好且经济高效的聚合物涂层石墨传感器。这种电位传感器适用于食品中ACM的直接原位分析，无需繁琐的样品预处理程序。该传感器基于ACM阳离子与四苯基硼酸钠（TPB）阴离子形成的离子缔合络合物作为离子交换位点成功开发，使用邻苯二甲酸二丁酯（DBP）作为增塑剂。该传感器在1×10至1×10 M的ACM宽浓度范围内表现出快速、稳定、选择性好且呈线性的能斯特响应（57.45 mV/十倍浓度变化），检测限为1×10 M。评估了该传感器的选择性行为、响应时间、寿命、pH工作范围和基本验证参数。与已发表的色谱方法相比，所开发的传感器能有效测定多种食品中的ACM含量。还对所开发的传感器进行了绿色度和白度评估，证实它是一个出色的绿色且经济高效的选择。此外，将所开发的传感器与最近发表的ACM传感器进行了统计学比较，以确保其最佳性能。
