Melissa Bisaccia, Elisa Binda, Gabriella Caruso, Maurizio Azzaro, Ombretta Dell' Acqua, Andrea Di Cesare, Eckert Ester Maria, Flavia Marinelli
Department of Biotechnology and Life Sciences (DBSV), University of Insubria, Varese, Italy.
National Research Council, Institute of Polar Sciences (ISP), Messina, Italy.
Environ Microbiol. 2025 Feb;27(2):e70045. doi: 10.1111/1462-2920.70045.
Applying both culture-independent and -dependent approaches, bacterial diversity of marine biofilm communities colonising polyvinyl chloride panels submerged in Terra Nova Bay (Ross Sea, Antarctica) was investigated. Panels were deployed in two sites subjected to a different degree of anthropogenic impact (Road Bay [RB] impacted site and Punta Stocchino [PTS] control site). Biofilm samples were collected after 3 or 12 months to evaluate both short- and long-term microbial colonisation. Taxonomic composition of the microbial community was studied by 16S rRNA gene amplicon sequencing. Proteobacteria was the predominant phylum, followed by Bacteroidetes, Actinobacteria, Verrucomicrobia and Firmicutes. Impacted RB biofilms were found to contain a relevant fraction of potentially pathogenic bacterial genera, accounting for 27.49% of the whole community. A total of 86 psychrotolerant bacterial strains were isolated from the biofilm samples using culture-dependent techniques designed to enrich in Actinobacteria. These strains were assigned to three different phyla: Actinobacteria (54.65%), Firmicutes (32.56%) and Proteobacteria (12.79%). 2.73% of genera identified by metabarcoding were recovered also through cultivation, while 11 additional genera were uniquely yielded by cultivation. Functional screening of the isolates revealed their hydrolytic and oxidative enzyme activity patterns, giving new insights into the metabolic and biotechnological potential of microbial biofilm communities in Terra Nova Bay seawater.
应用不依赖培养和依赖培养的方法，对定植于浸没在南极罗斯海特拉诺瓦湾的聚氯乙烯板上的海洋生物膜群落的细菌多样性进行了研究。这些板子部署在两个受到不同程度人为影响的地点（罗德湾[RB]受影响地点和斯托基诺角[PTS]对照地点）。在3个月或12个月后采集生物膜样本，以评估短期和长期的微生物定植情况。通过16S rRNA基因扩增子测序研究了微生物群落的分类组成。变形菌门是主要的门类，其次是拟杆菌门、放线菌门、疣微菌门和厚壁菌门。发现受影响的RB生物膜含有相当比例的潜在致病细菌属，占整个群落的27.49%。使用旨在富集放线菌的依赖培养技术，从生物膜样本中总共分离出86株耐冷细菌菌株。这些菌株被归为三个不同的门类：放线菌门（54.65%）、厚壁菌门（32.56%）和变形菌门（12.79%）。通过宏条形码鉴定的属中有2.73%也通过培养得到，同时培养还独特地产生了另外11个属。对分离菌株的功能筛选揭示了它们的水解和氧化酶活性模式，为特拉诺瓦湾海水中微生物生物膜群落的代谢和生物技术潜力提供了新的见解。
