Chen Jie, Shi Fangzheng, Chen Siya, Liu Yulu, Xu Yuxin
Department of Ophthalmology, Second Affiliated Hospital of Anhui Medical University, Hefei, People's Republic of China.
Department of Ophthalmology, Binhu District of Hefei First People's Hospital, People's Republic of China.
Indian J Ophthalmol. 2025 Jun 1;73(Suppl 3):S410-S417. doi: 10.4103/IJO.IJO_1314_24. Epub 2025 Apr 17.
Dysbiosis of the microbial community in the lacrimal sac has been associated with the occurrence and development of chronic dacryocystitis. The aim of this study was to investigate the microbial characteristics of the conjunctival sac and lacrimal sac in patients with chronic dacryocystitis using 16S rRNA sequencing and explore their relevance to this condition.
This was achieved through the application of Illumina MiSeq technology for the comprehensive sequencing of 16S rRNA genes in bacterial DNA samples isolated from the conjunctival and lacrimal sacs of 20 patients with chronic dacryocystitis.
The conjunctival sac microbiota showed slightly higher diversity compared to the lacrimal sac. However, their microbial communities were similar. In the cohort representing the lacrimal sac, there was a notable reduction in the relative abundance of Proteobacteria. Conversely, an increase in the relative richness of phyla such as Firmicutes, Actinobacteria, and Bacteroidetes was observed. Linear discriminant analysis and effect size analysis elucidated a significant enrichment of the Methylobacterium and Cetobacterium genera within the conjunctival sac cohort, in contrast to the lacrimal sac cohort. The dominant pathway in both sites was biosynthesis according to Kyoto Encyclopedia of Genes and Genomes analysis.
In individuals suffering from chronic dacryocystitis, the microbiota of the lacrimal sac exhibits a reduced α-diversity and a comparable β-diversity, alongside exhibiting distinct taxonomic profiles when contrasted with the microbiota of the conjunctival sac.
泪囊微生物群落失调与慢性泪囊炎的发生和发展有关。本研究的目的是使用16S rRNA测序研究慢性泪囊炎患者结膜囊和泪囊的微生物特征，并探讨它们与这种疾病的相关性。
通过应用Illumina MiSeq技术对从20例慢性泪囊炎患者的结膜囊和泪囊中分离的细菌DNA样本中的16S rRNA基因进行全面测序来实现这一目标。
结膜囊微生物群的多样性略高于泪囊。然而，它们的微生物群落相似。在代表泪囊的队列中，变形菌门的相对丰度显著降低。相反，观察到厚壁菌门、放线菌门和拟杆菌门等门的相对丰富度增加。线性判别分析和效应大小分析表明，与泪囊队列相比，结膜囊队列中的甲基杆菌属和鲸杆菌属显著富集。根据京都基因与基因组百科全书分析，两个部位的主要途径都是生物合成。
在患有慢性泪囊炎的个体中，泪囊微生物群的α多样性降低，β多样性相当，与结膜囊微生物群相比，其分类学特征也有所不同。
