Wu Zeyong, Shi Yucang, Zhang Bing, Liu Hongwei, Zhang Peihua
Department of Plastic Surgery, First Affiliated Hospital of Jinan University, Guangzhou 510632, China.
Department of Plastic Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524013, China.
ACS Omega. 2025 Jan 13;10(3):2986-2995. doi: 10.1021/acsomega.4c06103. eCollection 2025 Jan 28.
Natural jellyfish collagen (JC) has garnered significant attention in the field of hemostasis due to its oceanic origin, nontoxicity, biodegradability, and absence of complications related to diseases and religious beliefs. However, the hemostatic performance of pure JC is limited by its poor stability, adhesion to wet tissue, and mechanical properties. We developed a novel (HJP) sponge comprising JC, protocatechuic acid (PA), and hydroxybutyl chitosan (HS) to enhance the application of JC in emergency hemostasis. This sponge exhibits antibacterial properties, good biocompatibility, wet tissue adhesion, and hemostatic capabilities. The HJP sponge demonstrates excellent thermal stability and mechanical strength (tensile strength: ∼106.6 kPa, compressive strength at 70% compressive strain: ∼1013.5 kPa) and strong wet tissue adhesion (∼117.1 kPa). Upon application to a wound, the HJP sponge rapidly forms a wound seal, achieving effective hemostasis through the synergistic action of PA and JC. The blood loss was also reduced to 0.105 g when compared to a commercial gelatin sponge. This JC-based sponge, with its multifaceted characteristics, holds significant promise for rapid hemostasis in clinical applications.
天然水母胶原蛋白（JC）因其海洋来源、无毒、可生物降解以及不存在与疾病和宗教信仰相关的并发症，在止血领域受到了广泛关注。然而，纯JC的止血性能受到其稳定性差、对湿组织的粘附性以及机械性能的限制。我们开发了一种新型（HJP）海绵，它由JC、原儿茶酸（PA）和羟丁基壳聚糖（HS）组成，以增强JC在紧急止血中的应用。这种海绵具有抗菌性能、良好的生物相容性、对湿组织的粘附性以及止血能力。HJP海绵表现出优异的热稳定性和机械强度（拉伸强度：约106.6 kPa，70%压缩应变下的抗压强度：约1013.5 kPa）以及较强的对湿组织的粘附性（约117.1 kPa）。应用于伤口时，HJP海绵迅速形成伤口密封，通过PA和JC的协同作用实现有效止血。与商用明胶海绵相比，失血量也减少到了0.105 g。这种基于JC的海绵具有多方面的特性，在临床应用中快速止血方面具有巨大的潜力。
