通过捷径法从胶原纤维构建的双绿色止血海绵解体。 (原句表述似乎不太准确完整,可能影响理解)
Dual green hemostatic sponges constructed by collagen fibers disintegrated from by a shortcut method.
作者信息
Ding Cuicui, Cheng Kuan, Wang Yue, Yi Yifan, Chen Xiaohong, Li Jingyi, Liang Kaiwen, Zhang Min
机构信息
College of Ecological Environment and Urban Construction, Fujian University of Technology, Fuzhou, 350118, PR China.
Department of Ophthalmology, The 900th Hospital of Joint Logistic Support Force, PLA (Clinical Medical College of Fujian Medical University, Dongfang Hospital Affiliated to Xiamen University), Fuzhou, 350025, PR China.
出版信息
Mater Today Bio. 2024 Jan 7;24:100946. doi: 10.1016/j.mtbio.2024.100946. eCollection 2024 Feb.
Recently, biomacromolecules have received considerable attention in hemostatic materials. Collagen, an ideal candidate for hemostatic sponges due to its involvement in the clotting process, has been facing challenges in extraction from raw materials, which is time-consuming, expensive, and limited by cultural and religious restrictions associated with traditional livestock and poultry sources. To address these issues, this study explored a new shortcut method that using wild (HR), a marine fouling organism, as a raw material for developing HR collagen fiber sponge (HRCFs), which employed urea to disrupt hydrogen bonds between collagen fiber aggregates. This method simplifies traditional complex manufacturing processes while utilized marine waste, thus achieving dual green in terms of raw materials and manufacturing processes. FTIR results confirmed that the natural triple-helical structure of collagen was preserved. HRCFs exhibit a blood absorption ratio of 2000-3500 %, attributed to their microporous structure, as demonstrated by kinetic studies following a capillary model. Remarkably, the cytotoxicity and hemolysis ratio of HRCFs are negligible. Furthermore, during hemostasis tests using rabbit ear and kidney models, HRCFs significantly reduce blood loss and shorten hemostasis time compared to commercial gelatin sponge and gauze, benefiting from the capillary effect and collagen's coagulation activity. This study provides new insights into the design of collagen-based hemostatic biomaterials, especially in terms of both raw material and green manufacturing processes.
近年来,生物大分子在止血材料中受到了广泛关注。胶原蛋白因其参与凝血过程,是止血海绵的理想候选材料,但从原材料中提取胶原蛋白一直面临挑战,这一过程耗时、昂贵,且受到与传统畜禽来源相关的文化和宗教限制。为了解决这些问题,本研究探索了一种新的捷径方法,即使用野生(HR),一种海洋污损生物,作为开发HR胶原纤维海绵(HRCFs)的原材料,该方法采用尿素破坏胶原纤维聚集体之间的氢键。这种方法简化了传统的复杂制造工艺,同时利用了海洋废弃物,从而在原材料和制造工艺方面实现了双重绿色。傅里叶变换红外光谱(FTIR)结果证实,胶原蛋白的天然三螺旋结构得以保留。HRCFs的吸血率为2000-3500%,这归因于其微孔结构,毛细管模型的动力学研究证明了这一点。值得注意的是,HRCFs的细胞毒性和溶血率可以忽略不计。此外,在使用兔耳和肾脏模型的止血测试中,与商用明胶海绵和纱布相比,HRCFs显著减少了失血量并缩短了止血时间,这得益于毛细管效应和胶原蛋白的凝血活性。本研究为基于胶原蛋白的止血生物材料的设计提供了新的见解,特别是在原材料和绿色制造工艺方面。