Snari Razan M, Al-Ahmed Zehbah A, Alharbi Arwa, Sari Abdullah A A, Katouah Hanadi A, Shah Reem, Saad Fawaz A, Shaaban Fathy, El-Metwaly Nashwa M
Department of Chemistry, Faculty of Sciences, Umm Al-Qura University, Makkah 21955, Saudi Arabia.
Applied College Dhahran Aljanoub, King Khalid University, Abha 61421, Saudi Arabia.
ACS Omega. 2025 Jan 16;10(3):2897-2907. doi: 10.1021/acsomega.4c09110. eCollection 2025 Jan 28.
The present study described the fabrication and electroanalytical performance of planar screen-printed disposable sensors based on copper oxide nanoparticles (CuONPs/SPEs) for the voltammetric determination of tapentadol (TAP) in its marketed pharmaceutical products and biological fluids. CuONPs exhibited electrochemical activity against the electrooxidation of TAP with an anodic peak at 0.84 V in the BR buffer at pH 5. The electroanalytical investigations and molecular orbital calculations pointed out the oxidation of the tertiary amine group within the TAP moiety with the transfer of two electrons and protons during the oxidation of the TAP molecule at the CuONPs/SPEs surface. Linear calibration graphs were constructed covering the TAP concentration ranging from 0.83 to 738 ng mL with a limit of detection (LOD) value up to 0.24 ng mL. The disposable printed sensor based on CuONPs offered high measurement and fabrication reproducibility with a prolonged lifetime of 6 months. Improved performance toward TAP was recorded without noticeable interference from degradation products, additives, excipients, uric acid, and ascorbic acid. Moreover, tapentadol and paracetamol (PC) can be simultaneously quantified. The CuONPs/SPEs were applied for monitoring TAP residues and in vitro dissolution studies of TAP in commercial pharmaceutical formulations.
本研究描述了基于氧化铜纳米颗粒的平面丝网印刷一次性传感器（CuONPs/SPEs）的制备及其电分析性能，用于伏安法测定市售药品和生物流体中的喷他佐辛（TAP）。在pH 5的BR缓冲液中，CuONPs对TAP的电氧化表现出电化学活性，阳极峰出现在0.84 V。电分析研究和分子轨道计算指出，在CuONPs/SPEs表面TAP分子氧化过程中，TAP部分的叔胺基团发生氧化，转移两个电子和质子。构建了线性校准曲线，覆盖的TAP浓度范围为0.83至738 ng/mL，检测限（LOD）值高达0.24 ng/mL。基于CuONPs的一次性印刷传感器具有高测量和制备重现性，使用寿命长达6个月。记录到对TAP的性能改善，且不受降解产物、添加剂、辅料、尿酸和抗坏血酸的明显干扰。此外，喷他佐辛和对乙酰氨基酚（PC）可同时定量。CuONPs/SPEs用于监测TAP残留以及TAP在商业药物制剂中的体外溶出研究。
