Abumelha Hana M, Sayqal Ali, Snari Razan M, Alkhamis Kholood M, Alharbi Arwa, Al-Ahmed Zehbah A, El-Metwaly Nashwa M
Department of Chemistry, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
Department of Chemistry, College of Sciences, Umm Al-Qura University, Makkah 24230, Saudi Arabia.
ACS Omega. 2024 Mar 8;9(11):13458-13468. doi: 10.1021/acsomega.4c00370. eCollection 2024 Mar 19.
The present study introduced a novel disposable screen-printed carbon electrodes (SPCEs) modified with copper oxide/zeolite nanostructures for eco-friendly selective differential pulse voltammetric quantification of tetrahydrozoline (THZ) in eyedrop samples and biological fluids. Modification of the electrode matrix with copper oxide nanoparticles/zeolite nanostructures (CuONPs/ZY) with their effective and synergistic electrocatalytic activity enhanced the electrode performance against electrooxidation of THZ at 0.960 V in BR at pH 9.0 with a diffusion-controlled reaction mechanism. The tentative oxidation mechanism based on molecular orbital calculations postulates the oxidation of THZ molecules through oxidation of a nitrogen atom five-membered ring and the participation of two electrons/protons in the electrode reaction. Linear calibration curves were illustrated within a wide THZ concentration range from 0.24 to 57.2 μg mL recording a limit of detection (LOD) value of 0.0799 μg mL. The CuONPs/ZY/SPEs exhibited improved performance compared with the sole reported THZ sensor-based gold film-plated carbon paste electrodes, in addition to their high reproducibility of fabrication and measurement and prolonged shelf lifetime. Tetrahydrozoline was successfully assayed in the presence of excipients, degradation products, and chloramphenicol. The presented voltammetric sensor can be considered as an eco-friendly and reliable analytical approach for monitoring THZ residues in eye drop samples and biological fluids with high recovery compared with the official pharmacopeial analytical protocol. The presented sensors were assessed according to an EcoScale tool and also compared with the reported THZ sensor.
本研究介绍了一种新型一次性丝网印刷碳电极（SPCEs），该电极用氧化铜/沸石纳米结构进行了修饰，用于对眼药水样品和生物流体中的四氢唑啉（THZ）进行环保型选择性差分脉冲伏安法定量。用具有有效和协同电催化活性的氧化铜纳米颗粒/沸石纳米结构（CuONPs/ZY）修饰电极基体，增强了电极在pH 9.0的BR缓冲溶液中对THZ在0.960 V下进行电氧化的性能，其反应机理为扩散控制。基于分子轨道计算的初步氧化机理假定THZ分子通过五元氮原子环的氧化以及两个电子/质子参与电极反应而被氧化。在0.24至57.2 μg mL的宽THZ浓度范围内绘制了线性校准曲线，检测限（LOD）值为0.0799 μg mL。与唯一报道的基于THZ传感器的镀金碳糊电极相比，CuONPs/ZY/SPEs表现出更好的性能，此外还具有高的制备和测量重现性以及延长的保质期。四氢唑啉在辅料、降解产物和氯霉素存在的情况下成功进行了测定。与官方药典分析方法相比，所提出的伏安传感器可被视为一种环保且可靠的分析方法，用于监测眼药水样品和生物流体中的THZ残留，回收率高。所提出的传感器根据EcoScale工具进行了评估，并与报道的THZ传感器进行了比较。
