Khalid Minahil, Noreen Mamoona
Department of Microbiology and Molecular Genetics, The Women University Multan Mattital Campus Pakistan
RSC Adv. 2025 Jan 31;15(5):3172-3182. doi: 10.1039/d4ra03104k. eCollection 2025 Jan 29.
Medicinal plants contain phytochemicals that confer therapeutic potentials, allowing plants to perform various biologically significant functions. However, the therapeutic potential of these bioactive metabolites against multidrug-resistant bacteria is limited. Hence, the potential use of rhizopheric bacteria to isolate silver nanoparticles to enhance the potential of bioactive metabolites has been adopted by researchers. Therefore, this research aimed to biogenically produce silver nanoparticles using the rhizospheric soil of and to evaluate its impact in regulating the potential of bioactive metabolites produced from the medicinal plant . The powder extract of was macerated under four different environmental conditions including cold maceration, warm maceration, fermented, and unfermented for extraction of metabolites. Macerated extracts were then evaluated for phytochemical detection of bioactive metabolites like alkaloids, steroids, phenols, and saponins. Biogenic AgNPs were primarily confirmed by visible color change from colorless to brown and were further characterized using UV-vis spectroscopy, giving two absorbance peaks at 440 nm and 445 nm. Functional groups attached to biogenic AgNPs were detected by Fourier transform infrared (FTIR) analysis. The crystalline nature of biogenic AgNPs was evaluated by X-ray diffraction (XRD) giving a diffraction peak at angles of around 36°, 46°, 67°, and 77°. The particle size and morphological appearance of biogenic AgNPs were confirmed by Scanning Electron Microscopy (SEM). The total phenolic content of biogenic AgNPs and bioactive metabolites was estimated by the Folin-Ciocalteu (F-C) assay. The antimicrobial efficacy of biogenic AgNPs and bioactive metabolites against MDR bacterial strains was accessed. This research shows that biogenic AgNPs can be used as a strong agent in enhancing the antimicrobial potential of bioactive metabolites against MDR bacteria and they can be investigated for further experimental findings.
药用植物含有具有治疗潜力的植物化学物质，使植物能够发挥各种具有生物学意义的功能。然而，这些生物活性代谢物对多重耐药细菌的治疗潜力有限。因此，研究人员采用了利用根际细菌分离银纳米颗粒以增强生物活性代谢物潜力的方法。因此，本研究旨在利用[植物名称]的根际土壤生物合成银纳米颗粒，并评估其对调节该药用植物产生的生物活性代谢物潜力的影响。将[植物名称]的粉末提取物在四种不同的环境条件下进行浸渍，包括冷浸、温浸、发酵和未发酵，以提取代谢物。然后对浸渍提取物进行生物活性代谢物如生物碱、类固醇、酚类和皂苷的植物化学检测。生物合成的银纳米颗粒主要通过从无色到棕色的可见颜色变化得到证实，并进一步使用紫外可见光谱进行表征，在440nm和445nm处有两个吸收峰。通过傅里叶变换红外（FTIR）分析检测生物合成银纳米颗粒上附着的官能团。通过X射线衍射（XRD）评估生物合成银纳米颗粒的晶体性质，在约36°、46°、67°和77°的角度处有一个衍射峰。通过扫描电子显微镜（SEM）确认生物合成银纳米颗粒的粒径和形态外观。通过福林-西奥尔特（F-C）法测定生物合成银纳米颗粒和生物活性代谢物的总酚含量。评估了生物合成银纳米颗粒和生物活性代谢物对多重耐药细菌菌株的抗菌效果。本研究表明，生物合成银纳米颗粒可作为增强生物活性代谢物对多重耐药细菌抗菌潜力的强效剂，可进一步研究其实验结果。
