Department d'Estomatologia, Facultat de Medicina I Odontologia, Universitat de València, Valencia, Spain.
Department of Dermatology, Stomatology, Radiology and Physical Medicine, Faculty of Medicine, Morales Meseguer Hospital, University of Murcia, Murcia, Spain.
Int Endod J. 2022 Oct;55(10):1066-1080. doi: 10.1111/iej.13805. Epub 2022 Aug 11.
To assess the cytocompatibility and bioactive potential of the new calcium silicate cement-based sealer AH Plus Bioceramic Sealer (AHPbcs) on human periodontal ligament stem cells (hPDLSCs) compared with the epoxy resin-based sealer AH Plus (AHP) and the calcium silicate cement-based sealer Endosequence BC Sealer (ESbcs).
Standardized sample discs and 1:1, 1:2 and 1:4 eluates of the tested materials were prepared. The following assays were performed: surface element distribution via SEM-EDX, cell attachment and morphology via SEM, cell viability via a MTT assay, cell migration/proliferation via a wound-healing assay, osteo/cemento/odontogenic marker expression via RT-qPCR and cell mineralized nodule formation via Alizarin Red S staining. HPDLSCs were isolated from extracted third molars. Comparisons were made with hPDLSCs cultured in unconditioned (negative control) or osteogenic (positive control) culture media. Statistical significance was established at p < .05.
A higher peak of Ca + was detected from ESbcs compared with AHPbcs and AHP in SEM-EDX. Both AHPbcs and ESbcs showed significantly positive results in the cytocompatibility assays (cell viability, migration/proliferation, attachment and morphology) compared with a negative control group, whilst AHP showed significant negative results. Both AHPbcs and ESbcs exhibited an upregulation of at least one osteo/odonto/cementogenic marker compared with the negative and positive control groups. Both ESbcs and AHPbcs showed a significantly higher calcified nodule formation than the negative and positive control groups, indicative of their biomineralization potential and were also significantly higher than AHP group.
AH Plus Bioceramic Sealer exhibited a significantly higher cytocompatibility and bioactive potential than AH Plus and a similar cytocompatibility to that of Endosequence BC Sealer. Endosequence BC Sealer exhibited a significantly higher mineralization potential than the other tested sealers. The results from this in vitro study act as supporting evidence for the use of AH Plus Bioceramic Sealer in root canal treatment.
评估新型硅酸钙水泥基密封剂 AH Plus Bioceramic Sealer（AHPbcs）与人牙周膜干细胞（hPDLSCs）的细胞相容性和生物活性，与环氧树脂基密封剂 AH Plus（AHP）和硅酸钙水泥基密封剂 Endosequence BC Sealer（ESbcs）进行比较。
制备标准化的样本盘和测试材料的 1:1、1:2 和 1:4 浸提液。进行了以下检测：通过扫描电镜-能谱仪（SEM-EDX）检测表面元素分布，通过 SEM 观察细胞附着和形态，通过 MTT 测定法检测细胞活力，通过划痕愈合试验检测细胞迁移/增殖，通过 RT-qPCR 检测成骨/成牙/成牙骨质标志物的表达，通过茜素红 S 染色检测细胞矿化结节形成。从提取的第三磨牙中分离出人牙周膜干细胞。将其与未培养（阴性对照）或成骨培养（阳性对照）的 hPDLSCs 进行比较。在 p<.05 时认为具有统计学意义。
SEM-EDX 检测显示 ESbcs 中 Ca+峰值明显高于 AHPbcs 和 AHP。与阴性对照组相比，AHPbcs 和 ESbcs 在细胞相容性检测（细胞活力、迁移/增殖、附着和形态）中均表现出显著的阳性结果，而 AHP 则表现出显著的阴性结果。与阴性和阳性对照组相比，AHPbcs 和 ESbcs 至少上调了一个成骨/成牙/成牙骨质标志物。与阴性和阳性对照组相比，ESbcs 和 AHPbcs 的钙化结节形成明显更高，表明其具有生物矿化潜力，且明显高于 AHP 组。
AH Plus Bioceramic Sealer 的细胞相容性和生物活性明显高于 AH Plus，与 Endosequence BC Sealer 的细胞相容性相似。Endosequence BC Sealer 的矿化潜力明显高于其他测试的密封剂。这项体外研究的结果为 AH Plus Bioceramic Sealer 在根管治疗中的应用提供了支持证据。
