Ye Yong, Lu Xinke, Kong En, Wang Qianqian, Shen Lixiao, Zhong Shiwei, Wang Yiguang, Xiao Zheng, Deng Jinping, Zhao Hongbo, Dong Bin
School of Landscape Architecture, Zhejiang Agriculture and Forestry University, No. 666 Wusu Street, Lin'an District, Hangzhou 311300, Zhejiang, China.
Zhejiang Provincial Key Laboratory of Germplasm Innovation and Utilization for Garden Plants, Zhejiang Agriculture and Forestry University, No. 666 Wusu Street, Lin'an District, Hangzhou 311300, Zhejiang, China.
Hortic Res. 2024 Sep 24;12(1):uhae273. doi: 10.1093/hr/uhae273. eCollection 2025 Jan.
Ambient temperature is a pivotal factor in the regulation of the flowering process in plants. In this study, we found that high ambient temperature exerts an inhibitory effect on the flowering of "Sjigui". However, the underlying molecular mechanisms remain not fully understood. Through transcriptome analysis, a differently expressed C3H gene was identified, which is induced by high ambient temperature. OfC3H49 was demonstrated to delay the flowering process of and downregulate the expression of flowering-related genes in calli. Further investigation indicates that OfC3H49 as a transcriptional repressor directly suppresses the expression of the thereby causing a delay in flowering time. Furthermore, a WRKY transcription factor, OfWRKY17, was identified to be responsive to high ambient temperature, directly binding to the promoter and enhance expression. Overexpression of in resulted in a significant delay in flowering and induced the expression of in calli. Collectively, our findings delineate a regulatory module, OfWRKY17-OfC3H49, which is activated by high ambient temperature and functions as a negative regulator of flowering by suppressing the expression of in . This study provides novel insights into the molecular mechanisms involved in ambient temperature-mediated flowering control and contributes to the development of molecular breeding strategies for .
环境温度是植物开花过程调控中的一个关键因素。在本研究中，我们发现高环境温度对“四季桂”的开花具有抑制作用。然而，其潜在的分子机制仍未完全清楚。通过转录组分析，鉴定出一个差异表达的C3H基因，它受高环境温度诱导。已证明OfC3H49会延迟“四季桂”的开花进程，并下调愈伤组织中开花相关基因的表达。进一步研究表明，OfC3H49作为转录抑制因子直接抑制“四季桂”的表达，从而导致开花时间延迟。此外，还鉴定出一个WRKY转录因子OfWRKY17对高环境温度有响应，它直接结合“四季桂”的启动子并增强其表达。在“四季桂”中过表达OfWRKY17导致开花显著延迟，并在愈伤组织中诱导“四季桂”的表达。总的来说，我们的研究结果描绘了一个调控模块OfWRKY17 - OfC3H49，它由高环境温度激活，并通过抑制“四季桂”的表达作为开花的负调控因子。本研究为环境温度介导的开花控制所涉及的分子机制提供了新的见解，并有助于“四季桂”分子育种策略的发展。
