Wordinger R J, Orr E L, Pace K, Oakford L, Morrill A
J Reprod Fertil. 1985 Mar;73(2):451-6. doi: 10.1530/jrf.0.0730451.
The ovaries from mast cell-normal (+/+) and mast cell-deficient (W/Wv) mice were examined with light and electron microscopy. In addition the effect of ovariectomy and subsequent steroid treatment on total uterine histamine content, total mast cell numbers and surface and glandular epithelial cell heights was measured. The ovaries of +/+ mice were normal, displaying various stages of follicular growth and atresia and numerous corpora lutea; the ovaries of W/Wv mice lacked follicles and corpora lutea but contained numerous hyperplastic interstitial cells which contained numerous lipid droplets, vesiculated mitochondria and abundant endoplasmic reticulum suggestive of steroid synthesis. Steroid treatment of ovariectomized +/+ and W/Wv mice caused a significant increase in uterine wet weight and endometrial surface and glandular epithelial cell heights. In +/+ mice, steroid treatment caused a concomitant increase in total mast cells per uterine horn while mast cells were totally absent in W/Wv mice. The increase in uterine histamine in +/+ mice is consistent with the increase in mast cell numbers. Measurable amounts of uterine histamine, which increases slightly after steroid treatment, were demonstrated in W/Wv mice. Since the uteri of +/+ and W/Wv mice respond to steroids in a similar manner with the sole exception being histamine content and mast cell numbers, our results demonstrate the potential of using these animals to investigate the role(s) of uterine mast cells and non-mast cell uterine histamine in the process of implantation and the formation of a decidual cell response.
利用光学显微镜和电子显微镜对肥大细胞正常（+/+）和肥大细胞缺陷（W/Wv）小鼠的卵巢进行了检查。此外，还测定了卵巢切除及随后的类固醇治疗对子宫组胺总量、肥大细胞总数以及表面和腺上皮细胞高度的影响。+/+小鼠的卵巢正常，呈现出卵泡生长和闭锁的各个阶段以及大量黄体；W/Wv小鼠的卵巢缺乏卵泡和黄体，但含有大量增生的间质细胞，这些细胞含有大量脂滴、泡状线粒体和丰富的内质网，提示类固醇合成。对卵巢切除的+/+和W/Wv小鼠进行类固醇治疗，导致子宫湿重以及子宫内膜表面和腺上皮细胞高度显著增加。在+/+小鼠中，类固醇治疗导致每个子宫角的肥大细胞总数同时增加，而W/Wv小鼠中完全没有肥大细胞。+/+小鼠子宫组胺的增加与肥大细胞数量的增加一致。在W/Wv小鼠中也检测到了可测量的子宫组胺量，类固醇治疗后略有增加。由于+/+和W/Wv小鼠的子宫对类固醇的反应方式相似，唯一的例外是组胺含量和肥大细胞数量，我们的结果证明了利用这些动物来研究子宫肥大细胞和非肥大细胞子宫组胺在着床过程和蜕膜细胞反应形成中的作用的潜力。
