西高加索蝙蝠病毒和莱里达蝙蝠狂犬病病毒等不同狂犬病毒在欧洲的分布及肌肉致病性

European distribution and intramuscular pathogenicity of divergent lyssaviruses West Caucasian bat virus and Lleida bat lyssavirus.

作者信息

Leopardi Stefania, Dacheux Laurent, Serra-Cobo Jordi, Ábrahám Ágota, Bajić Branka, Bourhy Hervé, Bücs Szilárd-Lehel, Budinski Ivana, Castellan Martina, Drzewniokova Petra, Dundarova Heliana, Festa Francesca, Kergoat Lauriane, Leuchtmann Maxime, López-Roig Marc, Pontier Dominique, Priore Maria Francesca, Robardet Emmanuelle, Scaravelli Dino, Zecchin Barbara, Lanszki Zsófia, Görföl Tamás, Kemenesi Gábor, De Benedictis Paola

机构信息

Laboratory for Emerging Viral Zoonoses, Istituto Zooprofilattico Sperimentale delle Venezie, Padova, Italy.

Unit Lyssavirus, Epidemiology and Neuropathology, Institut Pasteur, Université Paris-Cité, Paris, France.

出版信息

iScience. 2025 Jan 2;28(2):111738. doi: 10.1016/j.isci.2024.111738. eCollection 2025 Feb 21.

DOI:10.1016/j.isci.2024.111738

PMID:39898037

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11787528/

Abstract

Among lyssaviruses, West Caucasian bat virus (WCBV) and Lleida bat lyssavirus (LLEBV) raise concern as their divergence from rabies virus leads to the inefficacy of available prophylactic agents. Both viruses were described in the bat . We investigated the European distribution of WCBV and LLEBV by screening sera from across eight countries, finding widespread serological evidence and positivity up to 70%. We evaluated the intramuscular lethality of wild type isolates in Syrian hamsters. WCBV induced 100% lethality and a clinical disease compatible with furious rabies. All animals infected with LLEBV remained healthy for 40 days, despite one individual testing positive in the brain. We confirmed LLEBV's intramuscular a-pathogenicity using mice. Infected hamsters developed antibodies by day seven, regardless the virus and the clinical outcome. This study highlights the widespread circulation of WCBV and LLEBV in Europe and suggests differences in neuro-invasiveness and/or pathogenesis that are crucial for risk assessment.

摘要

在狂犬病毒属病毒中，西高加索蝙蝠病毒（WCBV）和莱里达蝙蝠狂犬病毒（LLEBV）令人担忧，因为它们与狂犬病病毒的差异导致现有预防药物失效。这两种病毒均在蝙蝠中被发现。我们通过筛查来自八个国家的血清，对WCBV和LLEBV在欧洲的分布情况进行了调查，发现了广泛的血清学证据，阳性率高达70%。我们评估了野生型毒株对叙利亚仓鼠的肌肉内致死性。WCBV导致100%的致死率，并引发与狂暴型狂犬病相符的临床疾病。所有感染LLEBV的动物在40天内均保持健康，尽管有一只动物的脑部检测呈阳性。我们利用小鼠证实了LLEBV的肌肉内致病性。无论感染何种病毒以及临床结果如何，感染的仓鼠在第7天都会产生抗体。这项研究突出了WCBV和LLEBV在欧洲的广泛传播，并表明神经侵袭性和/或发病机制存在差异，这对风险评估至关重要。