来自全球及肝脏特异性Bmal1基因敲除小鼠肝脏组织的转录组数据集。

A Transcriptomic Dataset of Liver Tissues from Global and Liver-Specific Bmal1 Knockout Mice.

作者信息

Han Guohao, Li Dan, Zhang Haisen, Li Chao, Yang Luda, Ma Tiantian, Wang Xuerong, Ma Bairong, Wu Xiaodie, Tao Yang, Wang Ziang, Wang Aihua, Chao Hsu-Wen, Jin Yaping, Chen Huatao

机构信息

Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China.

Key Laboratory of Animal Biotechnology of the Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, 712100, China.

出版信息

Sci Data. 2025 Feb 3;12(1):199. doi: 10.1038/s41597-025-04545-4.

DOI:10.1038/s41597-025-04545-4

PMID:39900971

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11790919/

Abstract

The circadian clock regulates various physiological processes in mammals. The core circadian clock gene Bmal1 is crucial for maintaining the oscillations of the circadian clock system by controlling the rhythmic expression of numerous circadian clock-controlled genes. To explore the transcriptional changes associated with Bmal1 deletion in liver tissues, we collected liver tissues from global and liver-specific Bmal1 knockout mice, along with their respective control groups, at two circadian time points (CT2 and CT14) and used them for transcriptome sequencing analysis. Genotyping, locomotor activity analysis, and comprehensive quality control analyses, including base quality scores, GC content, and mapping rates, confirmed the high quality of sequencing data. Differential expression analysis and RT-qPCR validation confirmed the reliability and validity of the dataset. These data offer a valuable resource for researchers investigating the role of BMAL1 in liver physiology, pathology, and the broader field of circadian biology.

摘要

生物钟调节哺乳动物的各种生理过程。核心生物钟基因Bmal1通过控制众多生物钟控制基因的节律性表达，对维持生物钟系统的振荡至关重要。为了探索与肝脏组织中Bmal1缺失相关的转录变化，我们在两个生物钟时间点（CT2和CT14）从全局和肝脏特异性Bmal1基因敲除小鼠及其各自的对照组中收集肝脏组织，并将其用于转录组测序分析。基因分型、运动活动分析以及包括碱基质量分数、GC含量和比对率在内的全面质量控制分析，证实了测序数据的高质量。差异表达分析和RT-qPCR验证证实了数据集的可靠性和有效性。这些数据为研究人员研究BMAL1在肝脏生理学、病理学以及更广泛的生物钟生物学领域中的作用提供了宝贵的资源。