Jiang Zhen, Wei Yongqi, Wang Yun, Han Songjie, Li Ze, Liu Sihang, Wang Zihao, Li Zhijun, Feng Ting, Zhu Haiguang, Yuan Xun
College of Materials Science and Engineering, Qingdao University of Science and Technology (QUST), 53 Zhengzhou Rd., Shibei District, Qingdao 266042, P. R. China.
Department of Hematology, Qingdao Women and Children's Hospital, Qingdao 266011, P. R. China.
Nanoscale. 2025 Mar 7;17(10):5778-5787. doi: 10.1039/d4nr04718d.
Designing antibacterial agents with broad-spectrum antibacterial effects and resistance-free properties is essential for treating bacteriainfected wounds. In this study, we present the design of copper nanoclusters (Cu NCs) that exhibit aggregation-induced emission (AIE). This was achieved by controlling the aggregation state of ligand layers (cysteine and chitosan) through the manipulation of pH and temperature. The AIE properties, characterized by strong photoluminescence (PL), a large Stokes shift, and microsecond-long lifetimes, enable these Cu NCs to generate significant amounts of reactive oxygen species (ROS) upon light illumination for efficient bacterial elimination without inducing drug resistance. As a result, they effectively inactivate various microbial pathogens, including Gram-negative and Gram-positive bacteria, as well as (), achieving elimination rates of 99.52% for (), 98.89% for (), and 94.60% for . Furthermore, the natural antibacterial properties of chitosan and Cu species enhance the photodynamic antibacterial efficacy of the AIE-typed Cu NCs. Importantly, experiments demonstrate that these Cu NCs can effectively eradicate bacteria at infection sites, reduce inflammation, and promote collagen synthesis, facilitating nearly 100% wound recovery in -infected wounds within 9 days. The findings of this study are of considerable significance, providing a foundation for the application of AIE-typed Cu NCs in photodynamic nanotherapy for bacterial infections.
设计具有广谱抗菌作用且无耐药性的抗菌剂对于治疗细菌感染伤口至关重要。在本研究中，我们展示了具有聚集诱导发光（AIE）特性的铜纳米簇（Cu NCs）的设计。这是通过调节pH值和温度来控制配体层（半胱氨酸和壳聚糖）的聚集状态实现的。这些Cu NCs具有强烈的光致发光（PL）、大斯托克斯位移和微秒级寿命等AIE特性，使其在光照下能够产生大量活性氧（ROS）以有效消除细菌而不诱导耐药性。结果，它们能有效灭活各种微生物病原体，包括革兰氏阴性菌和革兰氏阳性菌，以及（此处原文括号内容缺失，无法准确翻译），对（此处原文括号内容缺失，无法准确翻译）的消除率为99.52%，对（此处原文括号内容缺失，无法准确翻译）的消除率为98.89%，对（此处原文括号内容缺失，无法准确翻译）的消除率为94.60%。此外，壳聚糖和铜物种的天然抗菌特性增强了AIE型Cu NCs的光动力抗菌效果。重要的是，（此处原文相关实验内容缺失，无法准确翻译）实验表明这些Cu NCs能够有效根除感染部位的细菌，减轻炎症，并促进胶原蛋白合成，在9天内使（此处原文相关感染伤口内容缺失，无法准确翻译）感染伤口的愈合率接近100%。本研究结果具有重要意义，为AIE型Cu NCs在细菌感染的光动力纳米治疗中的应用提供了基础。
