Wu Tao, Jiang Hanqiu, Lin Chen, Peng Jingting, Kong Xiuyun, Yu Jie, Wang Jiawei, Cui Shilei
Department of Clinical Nutrition, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
Department of Neurology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
J Int Med Res. 2025 Feb;53(2):3000605251314817. doi: 10.1177/03000605251314817.
To characterize the gut microbial composition of patients with optic neuritis (ON) or myasthenia gravis (MG).
Stool samples were collected from 45 patients with ON, 13 patients with MG, and 20 healthy controls. Microbial genomic DNA was extracted, and the V3-V4 regions of bacterial 16S rRNA genes were amplified and sequenced. Bioinformatic analyses was performed to compare the alpha-diversity, beta-diversity, taxonomic assignments, and bacterial richness of the groups. Differences in the abundances of microbial taxa were identified using linear discriminant analysis effect size (LEfSe) and analysis of variance.
Beta-diversity analysis showed distinct clustering of patient samples from the healthy controls. At the phylum and genus levels, , , , and predominated, but their proportions varied between groups. LEfSe analysis identified microbial taxa that were associated with each group. The patients showed lower abundances of certain intestinal probiotics, including , , and , than the controls. No significant differences were found between the disease subgroups. The family was significantly less abundant in the ON than in the MG group.
We have identified significant alterations in the gut microbiota of patients with ON or MG, and importantly, a notable reduction in intestinal probiotics.
描述视神经炎（ON）或重症肌无力（MG）患者的肠道微生物组成。
收集45例ON患者、13例MG患者和20名健康对照者的粪便样本。提取微生物基因组DNA，扩增并测序细菌16S rRNA基因的V3-V4区域。进行生物信息学分析以比较各组的α多样性、β多样性、分类归属和细菌丰富度。使用线性判别分析效应大小（LEfSe）和方差分析确定微生物类群丰度的差异。
β多样性分析显示患者样本与健康对照者明显聚类。在门和属水平上， 、 、 、 和 占主导，但它们在各组之间的比例有所不同。LEfSe分析确定了与每组相关的微生物类群。患者某些肠道益生菌（包括 、 和 ）的丰度低于对照组。疾病亚组之间未发现显著差异。ON组中 科的丰度明显低于MG组。
我们已经确定ON或MG患者的肠道微生物群有显著改变，重要的是，肠道益生菌显著减少。
