Du Jingfang, Yang Hong
State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Curr Res Food Sci. 2025 Jan 17;10:100975. doi: 10.1016/j.crfs.2025.100975. eCollection 2025.
2'-Fucosyllactose (2'-FL), one of the most representative oligosaccharides in human milk, is intimately linked to the enrichment of specific species. However, the efficacy of 2'-FL in modulating the probiotic responses of bifidobacterium has been rarely researched. Thereinto, three key issues have yet to be reported: the effects of 2'-FL hydrolysis on bifidobacterial growth, the protective effects of 2'-FL on bifidobacterium under gastrointestinal stress and the inhibitory activity of 2'-FL metabolites against spp. This work intended to address these concerns. 2'-FL dramatically accelerated the growth and proliferation of YH17 and BBI01. The glucose in lactose core on 2'-FL was preferable for to achieve substantial increases in biomass while the galactose was not readily available. Additionally, 2'-FL showed unique advantages in ameliorating the resistance of to gastrointestinal challenges. 2'-FL considerably improved the adhesive property of , thus facilitating the competitive elimination of ATCC 29544 and ATCC 51329 by B. The growth inhibition of 2'-FL on the strains was mediated by promoting the secretion of antibacterial substances from . The inhibitory activity hinged on the strains. 2'-FL specifically induced BBI01 to produce some antibacterial substances that were proteinaceous, thermostable and relatively stable even at pH 8.0. These antibacterial substances played a key role in the inhibitory activity and had a synergistic effect with acidification. These observations provide a useful guideline for developing synbiotic supplements to intervene the infant gut microbiota.
2'-岩藻糖基乳糖（2'-FL）是母乳中最具代表性的低聚糖之一，与特定物种的富集密切相关。然而，2'-FL调节双歧杆菌益生菌反应的功效鲜有研究。其中，有三个关键问题尚未见报道：2'-FL水解对双歧杆菌生长的影响、2'-FL在胃肠道应激下对双歧杆菌的保护作用以及2'-FL代谢产物对[具体菌种]的抑制活性。本研究旨在解决这些问题。2'-FL显著加速了[双歧杆菌菌株]YH17和[双歧杆菌菌株]BBI01的生长和增殖。2'-FL乳糖核心上的葡萄糖更有利于[双歧杆菌]实现生物量的大幅增加，而半乳糖则不易利用。此外，2'-FL在改善[双歧杆菌]对胃肠道挑战的抗性方面具有独特优势。2'-FL显著提高了[双歧杆菌]的黏附特性，从而促进了[双歧杆菌]对[大肠杆菌菌株]ATCC 29544和[金黄色葡萄球菌菌株]ATCC 51329的竞争性消除。2'-FL对[有害菌]菌株的生长抑制作用是通过促进[双歧杆菌]分泌抗菌物质介导的。抑制活性取决于[有害菌]菌株。2'-FL特异性诱导[双歧杆菌]BBI01产生一些抗菌物质，这些物质是蛋白质性质的，耐热，甚至在pH 8.0时也相对稳定。这些抗菌物质在抑制活性中起关键作用，并与酸化具有协同效应。这些观察结果为开发合生元补充剂以干预婴儿肠道微生物群提供了有用的指导。
