Kavitha Loganathan, Kuzhalmozhi Manogaran, Vijayashree Priyadharsini Jayaseelan, Arun Kumar Arunachalam, Umadevi Krishna Mohan Rao, Ranganathan Kannan
The Tamil Nadu Dr. MGR Medical University, Ragas Dental College and Hospital, Department of Oral and Maxillofacial Pathology, Chennai, India.
Aringnar Anna Memorial Cancer Research Institute, Chennai, India.
J Appl Oral Sci. 2025 Feb 3;33:e20240392. doi: 10.1590/1678-7757-2024-0392. eCollection 2025.
The oral cavity harbors a plethora of bacterial species. Dysbiosis of oral and gut microbiota is associated with several oral and systemic pathologies, such as cancer, obesity, diabetes, atherosclerosis and gastrointestinal diseases. Imbalance in the oral-gut microbial axis has been associated with head and neck squamous cell carcinoma (HNSCC). This study aims to analyze the bacterial profile of HNSCC across various taxonomic units, investigate molecular patterns associated with prevalent bacterial phylum in HNSCC, and compare the bacterial profile in HNSCC and gastrointestinal (GI) carcinoma using computational analysis.
The microbe-host transcriptomic, proteomic, and epigenetic analyses of HNSCC and GI carcinomas were performed using The Cancer Microbiome Atlas (TCMA) database. The differential expression of the host's mRNA transcripts and proteins associated with tumor microbiome were analyzed using The University of Alabama at Birmingham Cancer data analysis (UALCAN) and Clinical Proteomic Tumor Analysis Consortium (CPTAC) websites.
A decrease in Actinobacteria and an enrichment of Flavobacteria at the class level, Neisseriales, Pasteurellales, and Campylobacterales at the order level, Pasteurellaceae, Flavobacteriaceae, Campylobacteraceae, and Peptoniphilaceae at the family level, and Hemophilus, Porphyromonas, and Leptotrichia at the genus level were observed in HNSCC compared to the normal mucosa. RICTOR protein, mRNA transcripts (HIST1H2BB, SCARNA11, TBC1D21 gene), and hsa-miR-200a-5p miRNA were significantly correlated with prevalent bacterial species in HNSCC. A major increase in Actinobacteria, Fusobacteria, and Spirochaetes was observed in HNSCC compared to GI carcinoma.
The oral-gut microbial dysbiosis, as reflected by the differential abundance of bacterial species in oral and GI carcinomas, suggests the implication of tumor microbiome and their genomic interactions with the host in carcinogenesis.
口腔中存在大量细菌种类。口腔和肠道微生物群的失调与多种口腔和全身疾病相关，如癌症、肥胖、糖尿病、动脉粥样硬化和胃肠道疾病。口腔-肠道微生物轴的失衡与头颈部鳞状细胞癌（HNSCC）有关。本研究旨在分析HNSCC在不同分类单元中的细菌谱，研究与HNSCC中普遍存在的细菌门相关的分子模式，并使用计算分析比较HNSCC和胃肠道（GI）癌中的细菌谱。
使用癌症微生物组图谱（TCMA）数据库对HNSCC和GI癌进行微生物-宿主转录组学、蛋白质组学和表观遗传学分析。使用阿拉巴马大学伯明翰分校癌症数据分析（UALCAN）和临床蛋白质组肿瘤分析联盟（CPTAC）网站分析与肿瘤微生物组相关的宿主mRNA转录本和蛋白质的差异表达。
与正常黏膜相比，HNSCC中放线菌纲减少，黄杆菌纲、奈瑟菌目、巴斯德菌目和弯曲菌目在目水平上富集，巴斯德菌科、黄杆菌科、弯曲菌科和消化链球菌科在科水平上富集，嗜血杆菌属、卟啉单胞菌属和纤毛菌属在属水平上富集。RICTOR蛋白、mRNA转录本（HIST1H2BB、SCARNA11、TBC1D21基因）和hsa-miR-200a-5p miRNA与HNSCC中普遍存在的细菌种类显著相关。与GI癌相比，HNSCC中放线菌、梭杆菌和螺旋体显著增加。
口腔和GI癌中细菌种类的差异丰度反映出的口腔-肠道微生物失调表明肿瘤微生物组及其与宿主的基因组相互作用在致癌过程中的作用。
