Steele M K, Gallo R V, Ganong W F
Neuroendocrinology. 1985 Mar;40(3):210-6. doi: 10.1159/000124077.
The effects of intraventricular infusions of artificial cerebrospinal fluid (aCSF) or angiotensin II (AII) on LH secretion were investigated in rats that had been ovariectomized for 8 days. In untreated ovariectomized rats, the mean whole blood concentration of LH as well as the amplitude, frequency, and nadir of the LH pulses were not affected by infusion of aCSF or 15 ng AII/h, but were suppressed in a dose-dependent fashion by infusion of AII at doses of 150 or 600 ng/h. The AII receptor antagonist, saralasin, blocked the inhibitory effect of AII, demonstrating the specificity of the response to AII. In ovariectomized rats pretreated with estradiol, infusion of AII did not modify mean blood LH levels. However, in ovariectomized rats pretreated with both estradiol and progesterone, infusions of AII at 150 or 600 ng/h produced dose-dependent increases in mean LH concentrations. The results demonstrate both inhibitory and stimulatory effects of AII upon LH secretion, the direction of the effect being determined by gonadal steroids.
在已切除卵巢8天的大鼠中，研究了脑室内注入人工脑脊液（aCSF）或血管紧张素II（AII）对促黄体生成素（LH）分泌的影响。在未经处理的去卵巢大鼠中，注入aCSF或15 ng AII/小时对LH的平均全血浓度以及LH脉冲的幅度、频率和最低点没有影响，但注入150或600 ng/小时的AII会以剂量依赖的方式抑制这些指标。AII受体拮抗剂沙拉新可阻断AII的抑制作用，证明了对AII反应的特异性。在用雌二醇预处理的去卵巢大鼠中，注入AII不会改变平均血液LH水平。然而，在用雌二醇和孕酮预处理的去卵巢大鼠中，注入150或600 ng/小时的AII会使平均LH浓度产生剂量依赖性增加。结果表明AII对LH分泌具有抑制和刺激作用，其作用方向由性腺类固醇决定。
