Karakaya Emre, Aydin Fuat, Gümüşsoy Kadir Semih, Kayman Tuba, Güran Özgür, Güran Cansu, Yarim Doğancan, Gündüz Enes Said, Abay Seçil
Erciyes University, Faculty of Veterinary Medicine, Department of Microbiology, Kayseri, Turkey.
Erciyes University, Faculty of Veterinary Medicine, Department of Microbiology, Kayseri, Turkey.
Comp Immunol Microbiol Infect Dis. 2025 Apr;118:102314. doi: 10.1016/j.cimid.2025.102314. Epub 2025 Feb 3.
It was aimed at serotyping, genotyping, determining various virulence genes, and investigating antibiotic susceptibilities of Listeria monocytogenes isolates recovered from different sources in the current study. For this purpose, a total of 70 L. monocytogenes isolates including 22 chicken, 20 fish, 18 sheep, and 10 cattle origin were used. Polymerase Chain Reaction (PCR) was performed for serotyping and analysis of virulence genes of the isolates, and also Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR was performed for the genotyping. In addition, it was determined the susceptibilities of the isolates against nine different antibiotics via the disk diffusion method. As a result of serotyping, the most detected serogroup in analyzed L. monocytogenes isolates was 1/2a-3a (44.3 %), and but the least detected serogroup 1/2b-3b-7 (11.4 %). ERIC-PCR results revealed a total of 18 different patterns. All isolates were positive for the presence of inlA, inlB, inlC, iap, prfA, actA, hly, plcA, plcB and mpl virulence genes tested. The prevalence of the actA gene in isolates was determined as 70 %. Antibiotic resistance was detected against six antibiotics, and high resistance to oxacillin (80 %) and ciprofloxacin (65.7 %) in the isolates. Furthermore, the rate of multi-drug resistance in L. monocytogenes isolates was 28.5 % (20/70). In conclusion, the present study showed that the sources may pose a potential health risk, according to obtained data on the virulence gene prevalence, serogroup distribution, high genetic heterogeneity, and antibiotic resistance profiles of L. monocytogenes isolates from different sources.
本研究旨在对从不同来源分离出的单核细胞增生李斯特菌进行血清分型、基因分型、确定各种毒力基因，并研究其抗生素敏感性。为此，共使用了70株单核细胞增生李斯特菌分离株，包括22株来自鸡肉、20株来自鱼肉、18株来自羊肉和10株来自牛肉。采用聚合酶链反应（PCR）对分离株进行血清分型和毒力基因分析，同时采用肠杆菌重复基因间共有序列（ERIC）-PCR进行基因分型。此外，通过纸片扩散法测定了分离株对9种不同抗生素的敏感性。血清分型结果显示，在分析的单核细胞增生李斯特菌分离株中，最常检测到的血清群是1/2a-3a（44.3%），而最少检测到的血清群是1/2b-3b-7（11.4%）。ERIC-PCR结果显示共有18种不同的模式。所有检测的分离株中，inlA、inlB、inlC、iap、prfA、actA、hly、plcA、plcB和mpl毒力基因均呈阳性。分离株中actA基因的流行率为70%。检测到分离株对6种抗生素耐药，其中对苯唑西林（80%）和环丙沙星（65.7%）耐药性高。此外，单核细胞增生李斯特菌分离株的多重耐药率为28.5%（20/70）。总之，根据从不同来源的单核细胞增生李斯特菌分离株获得的毒力基因流行率、血清群分布、高遗传异质性和抗生素耐药谱数据，本研究表明这些来源可能构成潜在的健康风险。
