Wu Caiming, Zhou Siyu, Xie Changping, Chen Daipeng, Zheng Li
School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan Yazhou Bay Seed Laboratory, Hainan University, Sanya, 572025, China.
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, School of Tropical Agriculture and Forestry, Ministry of Education, Hainan University, Haikou, Hainan, 570228, China.
Arch Virol. 2025 Feb 8;170(3):50. doi: 10.1007/s00705-025-06219-3.
The full genome sequence of a positive-sense (+) single-stranded (ss) RNA virus, which we have named "Neopestalotiopsis nebuloides deltaflexivirus 1" (NnDFV1), from Neopestalotiopsis nebuloides strain N-7 was sequenced and analyzed. The NnDFV1 genome is 7,719 nucleotides in length with a GC content of 49%, excluding the poly(A) tail, and contains a large open reading frame (ORF1) and three smaller ORFs (2-4). ORF1 encodes a replication-associated polyprotein (RP) consisting of three conserved domains: viral methyltransferase (Mtr), viral helicase (Hel), and RNA-dependent RNA polymerase (RdRp), whereas ORFs 2-4 encode three hypothetical proteins (18-20 kDa). Phylogenetic analysis showed that NnDFV1 formed a distinct clade together with Pestalotiopsis deltaflexivirus 1 (PDFV1), which is a new member of the genus Deltaflexivirus within the family Deltaflexiviridae. This is the first report of a novel deltaflexivirus found in the phytopathogenic fungus Neopestalotiopsis nebuloides.
对来自拟盘多毛孢菌株N-7的一种正义（+）单链（ss）RNA病毒的全基因组序列进行了测序和分析，我们将其命名为“拟盘多毛孢星云状三角洲弯曲病毒1”（NnDFV1）。NnDFV1基因组长度为7719个核苷酸，GC含量为49%（不包括poly(A)尾），包含一个大的开放阅读框（ORF1）和三个较小的ORF（2-4）。ORF1编码一个由三个保守结构域组成的复制相关多聚蛋白（RP）：病毒甲基转移酶（Mtr）、病毒解旋酶（Hel）和RNA依赖性RNA聚合酶（RdRp），而ORF2-4编码三种假定蛋白（18-20 kDa）。系统发育分析表明，NnDFV1与盘多毛孢三角洲弯曲病毒1（PDFV1）一起形成了一个独特的进化枝，PDFV1是三角洲弯曲病毒科三角洲弯曲病毒属的一个新成员。这是在植物病原真菌拟盘多毛孢中发现新型三角洲弯曲病毒的首次报道。
