来自植物病原真菌胶孢炭疽菌的一种新型arna病毒的分子特征分析。

Molecular characterization of a novel narnavirus from the plant-pathogenic fungus Colletotrichum gloeosporioides.

作者信息

Luo Peiping, Lin Xuetuan, Zhou Siyu, Chen Daipeng, Sun Yinhui, Zheng Li

机构信息

State Key Laboratory of Tropical Crop Breeding, School of Breeding and Multiplication, Sanya Institute of Breeding and Multiplication), Hainan University, 572025, Sanya, Hainan, China.

出版信息

Arch Virol. 2025 Aug 25;170(9):200. doi: 10.1007/s00705-025-06391-6.

DOI:10.1007/s00705-025-06391-6

PMID:40855142

Abstract

Here, we characterized a novel mycovirus, Colletotrichum gloeosporioides narnavirus 1 (CgNV1), isolated from Colletotrichum gloeosporioides strain T-19, a fungal pathogen responsible for anthracnose in various crops. The CgNV1 genome consists of a 3,628-nucleotide-long positive-sense single-stranded RNA (+ ssRNA) with a GC content of 51.93% and has a single open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) of 1,155 amino acids (132.2 kDa). BLASTp analysis indicated that the CgNV1 RdRp shares the highest sequence identity (56.77%) with that of Erysiphe necator narnavirus 15. Phylogenetic analysis confirmed that CgNV1 is a distinct member of the family Narnaviridae. To our knowledge, this is the first report of a narnavirus infecting C. gloeosporioides, expanding the known diversity of mycoviruses associated with this phytopathogenic fungus.

摘要

在此，我们对一种从胶孢炭疽菌T-19菌株中分离出的新型真菌病毒——胶孢炭疽菌纳病毒1（CgNV1）进行了鉴定。胶孢炭疽菌T-19菌株是一种导致多种作物炭疽病的真菌病原体。CgNV1基因组由一段长度为3628个核苷酸的正链单链RNA（+ssRNA）组成，GC含量为51.93%，并且有一个单一的开放阅读框（ORF），编码一个由1155个氨基酸（132.2 kDa）组成的RNA依赖的RNA聚合酶（RdRp）。BLASTp分析表明，CgNV1的RdRp与葡萄白粉菌纳病毒15的RdRp具有最高的序列同一性（56.77%）。系统发育分析证实，CgNV1是纳病毒科的一个独特成员。据我们所知，这是关于一种感染胶孢炭疽菌的纳病毒的首次报道，扩展了与这种植物病原真菌相关的已知真菌病毒多样性。