Xu Ruiting, Chen Wenxi, Chen Sihui, Wang Xueshi, Xu Jin, Zhang Yuejin, Ma Yatuan
College of Chemistry and Pharmacy, Northwest A&F University, Yangling, Shaanxi, China.
Department of Ecological Environmental Engineering, Yangling Vocational and Technical College, Yangling, Shaanxi, China.
Mol Biotechnol. 2025 Feb 8. doi: 10.1007/s12033-025-01367-y.
This study investigated the microbial community composition and structure in healthy and diseased rhubarb (Rheum rhabarbarum) root systems, examining both root tissue and rhizosphere environments. Alpha diversity analysis revealed significantly higher microbial abundance in the rhizosphere compared to root tissues, with notable differences between healthy and diseased plants. Principal coordinate analysis demonstrated that bacterial community composition was primarily influenced by ecological niches (47.5% variation explained), whereas fungal communities segregated based on plant health status. Network analysis revealed increased bacterial community complexity in diseased plants rhizosphere (579 nodes, 13,016 edges) compared to healthy plants (542 nodes, 8700 edges), while fungal networks showed opposite trends with significant reduction in diseased conditions (147 nodes, 30 edges vs. 205 nodes, 418 edges). Correlation analysis identified significant associations between specific microbial taxa and soil properties, with notable positive correlations between certain bacteria (Oscillospirales) and fungi (Barnettozyma, Mortierella) with soil organic matter and nutrient availability. Pathogenic taxa, including Fusarium and members of Burkholderiales, showed negative correlations with beneficial microorganisms, suggesting potential antagonistic relationships. These findings provide crucial insights into the complex interactions within the rhubarb root microbiome and their implications for plant health, contributing to our understanding of root rot disease dynamics and potential management strategies.
本研究调查了健康和患病大黄（Rheum rhabarbarum）根系中的微生物群落组成和结构，同时考察了根组织和根际环境。α多样性分析表明，与根组织相比，根际中的微生物丰度显著更高，且健康植株和患病植株之间存在显著差异。主坐标分析表明，细菌群落组成主要受生态位影响（解释了47.5%的变异），而真菌群落则根据植株健康状况进行分离。网络分析显示，与健康植株（542个节点，8700条边）相比，患病植株根际的细菌群落复杂性增加（579个节点，13016条边），而真菌网络呈现相反趋势，在患病条件下显著减少（147个节点，30条边对比205个节点，418条边）。相关性分析确定了特定微生物类群与土壤性质之间的显著关联，某些细菌（颤螺菌目）和真菌（Barnettozyma、被孢霉属）与土壤有机质和养分有效性之间存在显著正相关。包括镰刀菌属和伯克霍尔德氏菌目的成员在内的致病类群与有益微生物呈负相关，表明可能存在拮抗关系。这些发现为大黄根际微生物组内的复杂相互作用及其对植株健康的影响提供了关键见解，有助于我们理解根腐病动态及潜在的管理策略。
