Ruyani Aceng, Suswati Emi, Pratiwi Dhea Prita, Elvia Rina, Oktoviani Oktoviani, Parlindungan Deni
Graduate School of Science Education, Bengkulu University, Bengkulu, Indonesia.
Conservation Education for Sustainability of Bio-Resources (CESB-R), Bengkulu University, Bengkulu, Indonesia.
Open Vet J. 2024 Dec;14(12):3296-3308. doi: 10.5455/OVJ.2024.v14.i12.14. Epub 2024 Dec 31.
Leaf ethanolic extract of (LE3H) has the potential to restore glucose, triglyceride, and uric acid disorders and reduce mercury toxicity. High levels of glucose (hyperglycemia), urine (uremia), and creatinine (hypercreatininemia) in the blood cause real health problems. Meanwhile, the phytochemical content in fruit ethanolic extract of (FE3H) is higher than that of LE3H.
This study evaluated the potential of FE3H as a solution for hyperglycemia, uremia, and hypercreatininemia in mice.
Quercetin levels in FE3H were determined by high-performance liquid chromatography. The first (A) stage used 25 male mice divided into five groups. On day (d)1, the body weight (BW) of the mice was weighed. On d2-11, 5-mg/gBW sucrose was given by gavage, and then, on d12, BW and blood glucose level of mice were determined. On d13, 0.52-mg/gBW glibenclamide was given in A2, 0.39-mg/gBW FE3H was given in A3, and 0.52-mg/g BW FE3H was given in A4 by gavage. Control, A0, was only given double-distilled water (DDW). On d14, BW and blood glucose levels of the mice were determined. The second (B) stage used 24 male mice divided into six groups. On d1, B1-B5 were injected intraperitoneally 5-mg/kg BW HgCl; then, on d3-5, they were given by gavage 0.2-mg/g BW Immunos in B2, 0.39-mg/gBW FE3H in B3, 0.52-mg/gBW FE3H in B4, and 0.65-mg/gBW FE3H in B5. Controls, B0 and B1, were only given DDW. On d6, the mice were killed by cervical dislocation. The weight of the kidney was determined, and then, the urea and creatinine levels were measured in blood samples from the heart.
FE3H contains 98.92 ± 12.88 µg/g quercetin. Sucrose tends to increase BW, and then, 0.39-mg/g BW FE3H treatment tends to restore BW close to control. Sucrose significantly increased blood glucose, and then, 0.39- and 0.52-mg/gBW FE3H treatment restored significantly blood glucose similar to control. HgCl increases kidney weight, and then, 0.65-mg/gBW FE3H treatment tends to restore kidney weight close to control. HgCl significantly increased urea and creatinine, and then, 0.52- and 0.65-mg/gBW FE3H treatment significantly restored urea and creatinine similar to the control.
FE3H, which is high in flavonoid quercetin, has the potential to restore hyperglycemia by 47.38%-48.18%, uremia by 29.04%-33.06%, and hypercreatininemia by 49.52%-54.28% in mice.
[植物名称]叶乙醇提取物（LE3H）有恢复葡萄糖、甘油三酯和尿酸紊乱以及降低汞毒性的潜力。血液中高血糖、高尿素（尿毒症）和高肌酐（高肌酐血症）会引发实际的健康问题。同时，[植物名称]果实乙醇提取物（FE3H）中的植物化学成分含量高于LE3H。
本研究评估FE3H作为小鼠高血糖、尿毒症和高肌酐血症解决方案的潜力。
采用高效液相色谱法测定FE3H中的槲皮素含量。第一（A）阶段使用25只雄性小鼠，分为五组。在第1天（d1），称取小鼠体重（BW）。在d2 - 11天，通过灌胃给予5 mg/gBW的蔗糖，然后在d12天，测定小鼠的体重和血糖水平。在d13天，A2组通过灌胃给予0.52 mg/gBW的格列本脲，A3组给予0.39 mg/gBW的FE3H，A4组给予0.52 mg/gBW的FE3H。对照组A0仅给予双蒸水（DDW）。在d14天，测定小鼠的体重和血糖水平。第二（B）阶段使用24只雄性小鼠，分为六组。在d1天，B1 - B5组腹腔注射5 mg/kgBW的HgCl₂；然后在d3 - 5天，B2组通过灌胃给予0.2 mg/gBW的免疫抑制剂，B3组给予0.39 mg/gBW的FE3H，B4组给予0.52 mg/gBW的FE3H，B5组给予0.65 mg/gBW的FE3H。对照组B0和B1仅给予DDW。在d6天，通过颈椎脱臼处死小鼠。测定肾脏重量，然后测定心脏血液样本中的尿素和肌酐水平。
FE3H含有98.92 ± 12.88 μg/g的槲皮素。蔗糖倾向于增加体重，然后，0.39 mg/gBW的FE3H处理倾向于使体重恢复至接近对照组水平。蔗糖显著增加血糖，然后，0.39 mg/gBW和0.52 mg/gBW的FE3H处理显著使血糖恢复至接近对照组水平。HgCl₂增加肾脏重量，然后，0.65 mg/gBW的FE3H处理倾向于使肾脏重量恢复至接近对照组水平。HgCl₂显著增加尿素和肌酐，然后，0.52 mg/gBW和0.65 mg/gBW的FE3H处理显著使尿素和肌酐恢复至接近对照组水平。
富含黄酮类槲皮素的FE3H有潜力使小鼠的高血糖恢复47.38% - 48.18%、尿毒症恢复29.04% - 33.06%、高肌酐血症恢复49.52% - 54.28%。
