Wang Zhaoxia, Huang Yanan, He Simin, Zhou Ying, Zhao Le, Wang Fuyuan
Department of Gynecology, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, People's Republic of China.
First Hospital of Shanxi Medical University, 85 South Jiefang Road, Taiyuan, Shanxi, 030001, P.R. China.
J Ovarian Res. 2025 Feb 10;18(1):25. doi: 10.1186/s13048-025-01608-3.
Exosomes, extracellular vesicles with an average diameter of 30 ~ 150 nm, are pivotal in mediating the cellular microenvironment (CM) through their cargo-carrying capability. Despite extensive studies, the dynamic and regulatory mechanisms of exosomal cargoes, including lipids, proteins, nucleic acids, and metabolites, remain poorly understood.
In this study, we collected culture medium of ovarian cancer cells at four different time points (12, 24, 36, 48 h). Exosomes were isolated using ultracentrifugation, and miRNA sequencing was performed for exosomes from each group (T12, T24, T36, and T48).
A total of 131 miRNAs were identified in all groups. Specifically, 41, 115, 63, and 24 miRNAs were detected in the T12, T24, T36, and T48 groups, respectively. Among these, 15 miRNAs were common to the all groups, while 3, 57, 10, and 3 miRNAs were unique to the T12, T24, T36, and T48 groups, respectively. Functional analyses of the target genes for both common and specific miRNAs indicated that numerous target genes were involved in signaling pathways and cancer-related processes.
It suggested that exosomal miRNAs might be critical in intercellular communication and in dynamically remodeling the tumor microenvironment. These insights could enhance our understanding of the role of exosomal miRNAs in cancer biology and inform the development of novel therapeutic strategies.
外泌体是平均直径为30至150纳米的细胞外囊泡，通过其携带货物的能力在介导细胞微环境（CM）中起关键作用。尽管进行了广泛的研究，但外泌体货物（包括脂质、蛋白质、核酸和代谢物）的动态和调节机制仍知之甚少。
在本研究中，我们在四个不同时间点（12、24、36、48小时）收集卵巢癌细胞的培养基。使用超速离心法分离外泌体，并对每组（T12、T24、T36和T48）的外泌体进行miRNA测序。
所有组共鉴定出131种miRNA。具体而言，在T12、T24、T36和T48组中分别检测到41、115、63和24种miRNA。其中，15种miRNA在所有组中都有，而3、57、10和3种miRNA分别是T12、T24、T36和T48组特有的。对常见和特定miRNA的靶基因进行功能分析表明，许多靶基因参与信号通路和癌症相关过程。
这表明外泌体miRNA可能在细胞间通讯和动态重塑肿瘤微环境中起关键作用。这些见解可以增强我们对外泌体miRNA在癌症生物学中的作用的理解，并为新型治疗策略的开发提供信息。
