Department of Pharmaceutics, College of Pharmacy, Jazan University, Jazan 45142, Saudi Arabia.
Department of Pharmaceutical Chemistry, College of Pharmacy, Jazan University, Jazan 45142, Saudi Arabia.
Molecules. 2022 Mar 9;27(6):1796. doi: 10.3390/molecules27061796.
The present study aimed to develop a local dental nanoemulgel formulation of oil (NSO) for the treatment of periodontal diseases. NSO purchased from a local market was characterized using a GC-MS technique. A nanoemulsion containing NSO was prepared and incorporated into a methylcellulose gel base to develop the nanoemulgel formulation. The developed formulation was optimized using a Box-Behnken statistical design (quadratic model) with 17 runs. The effects of independent factors, such as water, oil, and polymer concentrations, were studied on two dependent responses, pH and viscosity. The optimized formulation was further evaluated for droplet size, drug release, stability, and antimicrobial efficacy. The developed formulation had a pH of 7.37, viscosity of 2343 cp, and droplet size of 342 ± 36.6 nm. Sustained release of the drug from the gel for up to 8 h was observed, which followed Higuchi release kinetics with non-Fickian diffusion. The developed nanoemulgel formulation showed improved antimicrobial activity compared to the plain NSO. Given the increasing emergence of periodontal diseases and antimicrobial resistance, an effective formulation based on a natural antibacterial agent is warranted as a dental therapeutic agent.
本研究旨在开发一种局部口腔纳米乳凝胶制剂，用于治疗牙周病。从当地市场购买的 NSO 采用 GC-MS 技术进行了表征。制备含有 NSO 的纳米乳，并将其掺入甲基纤维素凝胶基质中，以开发纳米乳凝胶制剂。采用 Box-Behnken 统计设计（二次模型）进行优化，共进行了 17 次运行。研究了水、油和聚合物浓度等独立因素对两个依赖响应（pH 值和粘度）的影响。对优化后的制剂进行了粒径、药物释放、稳定性和抗菌功效的进一步评估。所开发的制剂的 pH 值为 7.37，粘度为 2343 cp，粒径为 342 ± 36.6 nm。观察到药物从凝胶中持续释放长达 8 小时，符合 Higuchi 释放动力学，具有非菲克扩散。与普通 NSO 相比，所开发的纳米乳凝胶制剂显示出增强的抗菌活性。鉴于牙周病和抗菌药物耐药性的日益增加，需要一种基于天然抗菌剂的有效制剂作为口腔治疗剂。
