Andersson B S, Jones D P
Anal Biochem. 1985 Apr;146(1):164-72. doi: 10.1016/0003-2697(85)90411-7.
A method to determine intracellular distribution of ions and metabolites under conditions of low oxygen concentration was developed. The technique involves rapid digitonin fractionation and addition of cyanide to inhibit reoxygenation. Applicability of the procedure was examined by studying the distribution of the lipophilic ion triphenylmethylphosphonium, the weak acid 5,5-dimethyloxazolidine-2,4-dione, and adenine nucleotides.
开发了一种在低氧浓度条件下测定离子和代谢物细胞内分布的方法。该技术包括快速洋地黄皂苷分级分离并添加氰化物以抑制再氧化。通过研究亲脂性离子三苯基甲基鏻、弱酸5,5-二甲基恶唑烷-2,4-二酮和腺嘌呤核苷酸的分布来检验该程序的适用性。
