Hoek J B, Nicholls D G, Williamson J R
J Biol Chem. 1980 Feb 25;255(4):1458-64.
The intracellular distribution of lipophilic ions and weak acids and bases was studied in intact hepatocytes as indicators of gradients of pH and membrane potential. Mitochondrial and plasma membrane potentials of 161 and 32.8 mV (negative inside), respectively, were calculated from the intracellular accumulation of triphenylmethylphosphonium (TPMP+) and thiocyanate in the intact hepatocyte, assuming a two-compartment model. The predominantly mitochondrial localization of TPMP+ was confirmed using disruption procedures that selectively break the plasma membrane but not the mitochondrial membrane. The intracellular distribution of 5,5-dimethyloxazolidine-2,4-dione and methylamine was studied with similar techniques. A cytosolic pH of 6.95 to 7.00 and a mitochondrial delta pH of 0.97 units was observed. From these results, a total mitochondrial protonmotive force in the intact cell in excess of -200 mV was obtained.
研究了完整肝细胞中亲脂性离子以及弱酸和弱碱的细胞内分布情况，以此作为pH梯度和膜电位的指标。假设为两室模型，根据完整肝细胞中三苯甲基鏻（TPMP +）和硫氰酸盐的细胞内积累情况，分别计算出线粒体膜电位和质膜电位为161 mV和32.8 mV（内侧为负）。使用选择性破坏质膜而非线粒体膜的破碎程序，证实了TPMP +主要定位于线粒体。采用类似技术研究了5,5 - 二甲基恶唑烷 - 2,4 - 二酮和甲胺的细胞内分布。观察到胞质pH为6.95至7.00，线粒体ΔpH为0.97个单位。根据这些结果，得出完整细胞中线粒体总质子动力超过 - 200 mV。
