Sharma Pankaj, Salunke Ankita, Pandya Nishi, Shah Hetvi, Pandya Parth, Parikh Pragna
Department of Zoology, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India, 390002.
Department of Biomedical and Life Sciences, School of Science, Navrachana University, Vadodara, Gujarat, India, 391410.
Sci Rep. 2025 Feb 12;15(1):5163. doi: 10.1038/s41598-025-89466-3.
The use of synthetic insecticides has been crucial in the management of insect pests however the extensive use of insecticides can result in the development of resistance. Callosobruchus chinensis is a highly destructive pest of stored grains, it's a major feeder and infests a range of stored grains that are vital to both global food security and human nutrition. We extensively investigated gene expression changes of adults in response to deltamethrin to decipher the mechanism behind the insecticide resistance. The analysis of gene expression revealed 25,343 unigenes with a mean length of 1,435 bp. All the expressed genes were identified, and analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment. Exposure to deltamethrin (4.6 ppm) causes 320 differentially expressed genes (DEGs), of which 280 down-regulated and 50 up-regulated. The transcriptome analysis revealed that DEGs were found to be enriched in pathways related to xenobiotics metabolism, signal transduction, cellular processes, organismal systems and information processing. The quantitative real-time PCR was used to validate the DEGs encoding metabolic detoxification. To the best of our knowledge, these results offer the first toxicity mechanisms enabling a more comprehensive comprehension of the action and detoxification of deltamethrin in C. chinensis.
合成杀虫剂的使用在害虫管理中至关重要，然而杀虫剂的广泛使用会导致抗性的产生。绿豆象是储存谷物的一种极具破坏性的害虫，它是主要的取食者，会侵害一系列对全球粮食安全和人类营养都至关重要的储存谷物。我们广泛研究了成虫对溴氰菊酯反应的基因表达变化，以解读抗杀虫剂背后的机制。基因表达分析揭示了25343个单基因，平均长度为1435bp。对所有表达的基因进行了鉴定，并通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）富集分析。暴露于溴氰菊酯（4.6ppm）导致320个差异表达基因（DEG），其中280个下调，50个上调。转录组分析表明，差异表达基因在与异生物质代谢、信号转导、细胞过程、生物系统和信息处理相关的途径中富集。使用定量实时PCR来验证编码代谢解毒的差异表达基因。据我们所知，这些结果首次提供了毒性机制，有助于更全面地理解溴氰菊酯在绿豆象中的作用和解毒过程。
