Lapid Roi, Motro Yair, Craddock Hillary, Salah Ikram, King Roni, Winner Katherine, Kahila Bar-Gal Gila, Moran-Gilad Jacob
The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel.
Department of Health Policy and Management, School of Public Health, Faculty of Health Sciences, Ben-Gurion University of the Negev, Be'er-Sheva, Israel.
mSphere. 2025 Mar 25;10(3):e0081924. doi: 10.1128/msphere.00819-24. Epub 2025 Feb 13.
The spread of antimicrobial resistance (AMR) is a critical One Health issue. Wildlife could act as reservoirs or vehicles of AMR bacteria (ARBs) and AMR genes (ARGs) but are relatively understudied. We sought to investigate clinically relevant ARGs in golden jackals () thriving near human settlements in Israel. Fecal samples were collected from 111 jackals across four regions over a 10-month period. Various animal and spatio-temporal metadata were collected. Samples were analyzed by quantitative PCR (qPCR) for beta-lactamases (TEM, CTX-M15, and SHV), and . A subset of samples was subject to shotgun metagenomic sequencing followed by resistome and microbiome analyses. qPCR detected a high prevalence of ARGs, including beta-lactamases (TEM-1, 96.4%; CTX-M-15, 51.4%, SHV, 15.3%), fluoroquinolone resistance (, 87.4%), and class 1 integrons (, 94.6%). The TEM-1 gene was found to be more prevalent in adult jackals compared to younger ones. Metagenomic analysis of a subset of samples revealed a diverse gut microbiome harboring a rich resistome with tetracycline resistance genes being the most prevalent. Metagenome-assembled genome analysis further identified several ARGs associated with clinically relevant bacteria. These findings highlight the potential role of golden jackals as reservoirs for AMR and emphasize the need for ongoing surveillance to better understand AMR transmission dynamics at the wildlife-human interface.
The research highlights the potential role of the golden jackals as reservoirs for antimicrobial resistance (AMR). The high prevalence of clinically relevant AMR genes in these jackals emphasizes the need for ongoing surveillance and monitoring to better understand AMR transmission dynamics at the wildlife-human interface.
抗菌药物耐药性（AMR）的传播是一个关键的“同一健康”问题。野生动物可能充当AMR细菌（ARB）和AMR基因（ARG）的储存库或传播媒介，但相关研究相对较少。我们试图调查在以色列人类住区附近繁衍生息的金豺（）体内与临床相关的ARG。在10个月的时间里，从四个地区的111只金豺身上采集了粪便样本。收集了各种动物以及时空元数据。通过定量PCR（qPCR）分析样本中的β-内酰胺酶（TEM、CTX-M15和SHV）、 以及 。对一部分样本进行鸟枪法宏基因组测序，随后进行耐药基因组和微生物组分析。qPCR检测到ARG的高流行率，包括β-内酰胺酶（TEM-1，96.4%；CTX-M-15，51.4%，SHV，15.3%）、氟喹诺酮耐药性（ ，87.4%）和1类整合子（ ，94.6%）。发现TEM-1基因在成年金豺中比在幼年金豺中更普遍。对一部分样本的宏基因组分析揭示了一个多样的肠道微生物组，其中含有丰富的耐药基因组，四环素耐药基因最为普遍。宏基因组组装基因组分析进一步确定了几个与临床相关细菌相关的ARG。这些发现突出了金豺作为AMR储存库的潜在作用，并强调需要持续监测，以更好地了解野生动物与人类界面处的AMR传播动态。
该研究突出了金豺作为抗菌药物耐药性（AMR）储存库的潜在作用。这些金豺体内与临床相关的AMR基因的高流行率强调了持续监测的必要性，以便更好地了解野生动物与人类界面处的AMR传播动态。
