意大利中部地区金豺（Canis aureus L. 1758）中的抗菌药物耐药基因。
Antimicrobial resistance genes in a golden jackal (Canis aureus L. 1758) from Central Italy.
机构信息
Department of Veterinary Medical Sciences (DIMEVET), University of Bologna, Ozzano dell' Emilia (BO), Italy.
Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche 'Togo Rosati', Perugia, Italy.
出版信息
Vet Res Commun. 2023 Dec;47(4):2351-2355. doi: 10.1007/s11259-023-10172-4. Epub 2023 Jul 12.
In recent years an increasing interest has been focused on the contribution of wildlife in ecology and evolution of the antimicrobial resistance (AMR). The aim of this study was to molecularly investigate the presence of antimicrobial resistance genes (ARGs) in organ samples from a golden jackal (Canis aureus) found dead in the Marche region (Central Italy). Samples from lung, liver, spleen, kidney, and intestine were investigated by PCRs targeting the following genes: tet(A), tet(B), tet(C), tet(D), tet(E), tet(G), tet(K), tet(L), tet(M), tet(O), tet(S), tet(P), tet(Q), tet(X), sul1, sul2, sul3, bla, bla, bla, and mcr-1 to mcr-10. One or more ARGs were detected in all organs tested, except the spleen. Specifically, the lung and liver were positive for tet(M) and tet(P), the kidney for mcr-1 and the intestine for tet(A), tet(L), tet(M), tet(O), tet(P), sul3 and bla. These results, according to the opportunistic foraging strategy of the jackal, confirm its potential role as a good bioindicator of AMR environmental contamination.
近年来，人们对野生动物在抗菌药物耐药性（AMR）的生态学和进化中的贡献越来越感兴趣。本研究旨在通过针对以下基因的 PCR 检测，从在意大利中部马尔凯地区发现的死亡金豺（Canis aureus）的器官样本中分子检测抗菌药物耐药基因（ARGs）的存在：tet(A)、tet(B)、tet(C)、tet(D)、tet(E)、tet(G)、tet(K)、tet(L)、tet(M)、tet(O)、tet(S)、tet(P)、tet(Q)、tet(X)、sul1、sul2、sul3、bla、bla、bla 和 mcr-1 到 mcr-10。除了脾脏，所有检测的器官都检测到了一个或多个 ARGs。具体来说，肺和肝对 tet(M)和 tet(P)呈阳性，肾对 mcr-1 呈阳性，肠对 tet(A)、tet(L)、tet(M)、tet(O)、tet(P)、sul3 和 bla 呈阳性。根据豺的机会主义觅食策略，这些结果证实了它作为 AMR 环境污染的良好生物指示剂的潜在作用。
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