Shojaeian Shiva, Asnaashari Mohamad, Heidari Arash, Sadeghi Mahsa, Mehrabinia Pegah
Department of Endodontics, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2024 Dec 28;15:e70. doi: 10.34172/jlms.2024.70. eCollection 2024.
Photodynamic therapy(PDT)is a minimally invasive technique increasingly used in dentistry for its antimicrobial properties. This research aimed to evaluate the influence of photodynamic therapy (PDT) on the viability of dental pulp stem cells (DPSCs). In this laboratory-based, experimental study, DPSCs were cultured in Dulbecco's modified Eagle's medium and maintained at 37 °C. The cells were separated into five groups: Toluidine blue (TBO) at concentration of 0.1 mg/mL and 0.5 mg/mL, as well as methylene blue (MB) at concentrations of 0.01 mg/mL and 0.05 mg/mL were added to the wells in groups 1 to 4. The fifth group served as the control group. After 5 minutes of incubation, the experimental groups were irradiated with Fotosan® light-emitting diode (LED) for one minute. Cell viability was assessed after 8, 24, 48, and 72 hours using the methyl thiazolyl tetrazolium (MTT) assay. Time (<0.000), photosensitizer type/concentration (<0.0001), and their interaction effect (<0.000) on cell viability were all significant. Viability in both MB groups was considerably higher than that in the control group at 8 hours (<0.001). At 24 hours, no significant difference was observed between the experimental groups and the control (>0.05). At 48 and 72 hours, cell viability in the TBO groups was markedly lower compared to the control group (<0.01). PDT with MB at the tested concentrations had no adverse effect on DPSCs even in the long- term (48 and 72 hours).
光动力疗法（PDT）是一种微创技术，因其抗菌特性而在牙科领域越来越多地被使用。本研究旨在评估光动力疗法（PDT）对牙髓干细胞（DPSCs）活力的影响。在这项基于实验室的实验研究中，将DPSCs培养于杜尔贝科改良伊格尔培养基中，并在37℃下保存。将细胞分为五组：第1至4组的孔中分别加入浓度为0.1mg/mL和0.5mg/mL的甲苯胺蓝（TBO）以及浓度为0.01mg/mL和0.05mg/mL的亚甲蓝（MB）。第五组作为对照组。孵育5分钟后，对实验组用Fotosan®发光二极管（LED）照射1分钟。使用噻唑蓝（MTT）法在8、24、48和72小时后评估细胞活力。时间（<0.000）、光敏剂类型/浓度（<0.0001）及其对细胞活力的交互作用（<0.000）均具有显著意义。在8小时时，两个MB组的活力均显著高于对照组（<0.001）。在24小时时，实验组与对照组之间未观察到显著差异（>0.05）。在48和72小时时，TBO组的细胞活力明显低于对照组（<0.01）。在所测试浓度下，MB进行的PDT即使在长期（48和72小时）对DPSCs也没有不良影响。
