Yang Wei, Li Xiaonan, Zhang Mengjie, Wang Qi, Wang Yajun, Yu Susu, Dang Ruhua, Wang Xinru, Yang Zheng, Fan Suhua, Wu Hai, Wei Bing
School of Chemistry and Materials Engineering, Anhui Provincial Key Laboratory of Green Carbon Chemistry, Engineering Research Center of Biomass Conversion and Pollution Prevention of Anhui Educational Institutions, Biomass Oligosaccharides Engineering Technology Research Center of Anhui Province, Fuyang Normal University, Fuyang 236037, PR China.
School of Chemistry and Materials Engineering, Anhui Provincial Key Laboratory of Green Carbon Chemistry, Engineering Research Center of Biomass Conversion and Pollution Prevention of Anhui Educational Institutions, Biomass Oligosaccharides Engineering Technology Research Center of Anhui Province, Fuyang Normal University, Fuyang 236037, PR China; Research Center of Anti-aging Chinese Herbal Medicine of Anhui Province, Biology and Food Engineering School, Fuyang Normal University, Fuyang 236037, PR China.
Food Chem. 2025 May 30;475:143397. doi: 10.1016/j.foodchem.2025.143397. Epub 2025 Feb 12.
Mycotoxin contamination poses a great threat to food safety and human health. Thus, universal and sensitive detection of mycotoxins is urgently needed. Herein, N/O co-doped porous biomass carbon was synthesized from rice straw as a novel electrode modification material for fabricating an electrochemical sensor for mycotoxin detection. The fabricated sensor exhibited excellent universality in the detection of aflatoxin B1, aflatoxin G1, aflatoxin G2, aflatoxin M1, zearalenone, and deoxynivalenol. The limits of detection were ca. 0.5689, 0.0504, 0.0274, 0.6141, 0.0781, and 0.0512 fg·mL, respectively. The dynamic linear range was spanned from 0.001 to 1000 pg·mL. The biomass carbon-based electrochemical sensor also demonstrated accurate and rapid performance in detecting mycotoxins in real samples, with all recoveries near 100 %. Density functional theory calculation confirmed that the adsorption of mycotoxins by porous carbon changed the charge distribution of the electrode surface, which is the potential working mechanism of the designed electrochemical sensor for high sensitivity mycotoxin detection. The results indicated that the high sensitivity of the fabricated electrochemical sensor makes it suitable for the fast and accurate detection of mycotoxins in grain and feed products.
霉菌毒素污染对食品安全和人类健康构成了巨大威胁。因此，迫切需要对霉菌毒素进行通用且灵敏的检测。在此，以稻草为原料合成了氮/氧共掺杂多孔生物质碳，作为一种新型电极修饰材料用于制备检测霉菌毒素的电化学传感器。所制备的传感器在检测黄曲霉毒素B1、黄曲霉毒素G1、黄曲霉毒素G2、黄曲霉毒素M1、玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇时表现出优异的通用性。检测限分别约为0.5689、0.0504、0.0274、0.6141、0.0781和0.0512 fg·mL。动态线性范围为0.001至1000 pg·mL。基于生物质碳的电化学传感器在实际样品中检测霉菌毒素时也表现出准确快速的性能，所有回收率均接近100%。密度泛函理论计算证实，多孔碳对霉菌毒素的吸附改变了电极表面的电荷分布，这是所设计的用于高灵敏度检测霉菌毒素的电化学传感器的潜在工作机制。结果表明，所制备的电化学传感器的高灵敏度使其适用于谷物和饲料产品中霉菌毒素的快速准确检测。
