Intraoperative neuronavigation is currently an essential component of neurosurgical operations in several contexts. Recent progress in mixed reality (MR) technology has attempted to overcome the disadvantages of standard neuronavigation systems allowing the surgeon to superimpose a 3D rendered image onto the patient's anatomy. We present the first study in the literature to assess the surface matching accuracy of MR rendered image. For the purposes of this study, we used HoloLens 2 with virtual surgery intelligence providing the software capability for image rendering. To assess the accuracy of using mixed reality device for neuronavigation intraoperatively. This study seeks to assess the accuracy of rendered holographic images from a mixed reality device as a means for neuronavigation intraoperatively. We used the Realistic Operative Workstation for Educating Neurosurgical Apprentices to represent a patient's skull with intracranial components which underwent standardized computed tomography (CT) and MRI imaging. Eleven predefined points were used for purposes of assessing the accuracy of the rendered image, compared with the intraoperative gold standard neuronavigation. The mean HoloLens values against the ground truth were significantly higher when compared with Stealth using CT scan as the imaging modality. Using extracranial anatomic landmarks, the HoloLens error values continued to be significantly higher in magnitude when compared with Stealth across CT and MRI. This study provides a relatively easy and feasible method to assess accuracy of MR-based navigation without requiring any additions to the established imaging protocols. We failed to show the equivalence of MR-based navigation over the current neuronavigation systems.