Callow R A, McGrath J J
Cryobiology. 1985 Jun;22(3):251-67. doi: 10.1016/0011-2240(85)90146-4.
Cryomicroscope studies of large unilamellar liposomes indicate that liposomes are an excellent model for studying membrane response to freezing and thawing. Liposomes are attractive for such use because they can be custom-manufactured for a particular investigation. In addition, liposome responses to freezing and thawing mimic real cell behavior in a number of significant ways. Analogous behavior includes osmotic shrinkage at slow cooling rates, internal ice formation at fast cooling rates, comparable nucleation temperatures, and a variety of comparable thawing responses. Experimental determination has been made of the equilibrium osmotic properties and the nonequilibrium water transport properties of the egg lecithin liposomes used in the freezing studies. These properties have been used in a computer model to simulate volume changes resulting from water transport during freezing and thawing. Comparison between computer model predictions and experimental data for the liposome volume response during freezing indicates reasonable agreement whereas computer simulations of volume response during thawing do not match experimental data well.
对大单层脂质体的低温显微镜研究表明，脂质体是研究膜对冻融反应的极佳模型。脂质体因其可针对特定研究定制生产而适用于此类用途。此外，脂质体对冻融的反应在许多重要方面模拟了真实细胞的行为。类似行为包括在缓慢冷却速率下的渗透收缩、在快速冷却速率下的内部结冰、可比的成核温度以及各种可比的解冻反应。已对冷冻研究中使用的卵磷脂脂质体的平衡渗透性质和非平衡水传输性质进行了实验测定。这些性质已被用于计算机模型，以模拟冻融过程中因水传输导致的体积变化。冷冻期间脂质体体积反应的计算机模型预测与实验数据之间的比较表明具有合理的一致性，而解冻期间体积反应的计算机模拟与实验数据不太匹配。
