Uysal Çalıcıoğlu Pelin, Akpınar Fatma, Gençdağ Şensoy Kübra, Çalışkan Şerife Gökçe, Muti Mihrican
Department of Chemistry, Faculty of Science, Aydın Adnan Menderes University, Aydın, 09010, Turkey.
Department of Food Processing, Köşk Vocational High School, Aydın Adnan Menderes University, Aydın, 09570, Turkey.
Anal Sci. 2025 Jun;41(6):783-792. doi: 10.1007/s44211-025-00727-7. Epub 2025 Feb 17.
Infrared (IR) rays released from natural and artificial sources reach the layers of the skin and cause different effects. The most important effects of IR rays are the deterioration of the structure of proteins in the skin and the inadequacy of the skin's defense system. Early diagnosis of DNA damage caused by IR rays and discovery of products with antioxidant properties that will reduce the effects of damage are important in the prevention and treatment of possible diseases. This study aimed to determine the harmful effects of IR rays on DNA by measuring the guanine oxidation signal. Within the scope of the study, electrodes with DNA immobilized on their surfaces were exposed to IR irradiation and the change in guanine oxidation was detected electrochemically. A 57% decrease in guanine oxidation signal was observed as a result of IR exposure, and this rate decreased in the presence of chitosan. The results obtained show that chitosan is effective in preventing DNA damage caused by IR rays and suggest that chitosan-supported products can be used to protect the skin from the harmful effects of sun rays. Furthermore, molecular docking analyses indicated no interaction between chitosan and collagen protein. This suggests that the addition of chitosan to any cosmetic product applied to the skin is unlikely to induce DNA damage attributable to chitosan.
天然和人工来源释放的红外线（IR）会到达皮肤各层并产生不同影响。红外线最重要的影响是皮肤中蛋白质结构的恶化以及皮肤防御系统的不足。早期诊断红外线引起的DNA损伤并发现具有抗氧化特性、能减轻损伤影响的产品，对于预防和治疗可能出现的疾病至关重要。本研究旨在通过测量鸟嘌呤氧化信号来确定红外线对DNA的有害影响。在该研究范围内，将表面固定有DNA的电极暴露于红外辐射下，并通过电化学方法检测鸟嘌呤氧化的变化。结果发现，红外照射导致鸟嘌呤氧化信号降低了57%，而在壳聚糖存在的情况下，该速率有所下降。所得结果表明壳聚糖在预防红外线引起的DNA损伤方面有效，并表明壳聚糖负载的产品可用于保护皮肤免受阳光射线的有害影响。此外，分子对接分析表明壳聚糖与胶原蛋白之间没有相互作用。这表明在应用于皮肤的任何化妆品中添加壳聚糖不太可能引发由壳聚糖导致的DNA损伤。
