Mohammed Mona A, El-Gengaihi Souad E, Maklad Yousreya A, Shabana Marwa E, Naeim Attia Hanan
Medicinal and Aromatic Plants Research Department, Pharmaceutical Industries Research Institute, National Research Centre, Giza, Egypt.
Medicinal and Pharmaceutical Chemistry Department (Pharmacology Group), Pharmaceutical Industries Research Institute, National Research Centre, Giza, Egypt.
Sci Rep. 2025 Feb 17;15(1):5780. doi: 10.1038/s41598-024-81874-1.
Opuntia ficus-indica red fruit (OFI-RF) is a member of the Cactaceae family and native to South America. Phytochemical evaluation of the plant has revealed variable bioactive components; therefore, this study explored the medicinal value of butanol (BE) and ethylacetate extracts (EE) by evaluating their antidiabetic, antioxidant and antihypercholesterolemic properties. Selected solvents were used for phytochemical extraction according to established protocols, and then pharmacological effects of phenolic and betanin-rich extracts were evaluated. Results indicated that butanol was the most effective solvent for extracting polyphenolics followed by ethyl acetate, yielding: 148.91 ± 0.95 and 110.96 ± 0.61 μg/g, respectively. Identification analysis of OFI-RF using UPLC/HESI-MS/MS revealed a diverse range of 101 metabolites, including polyphenolics (phenolic acids, phenolic glycosides, flavanols, flavanonols, flavonoids and biflavonoids), alkaloids, pyridine, betalains, coumarins, vitamins, fatty acids and other therapeutic compounds. Biological studies (in vitro and in vivo) demonstrated that both EE and BE exhibited significant antidiabetic, antioxidant and antihypercholestremic activities. These findings were further supported via histopathological examination.
仙人掌科胭脂仙人掌红色果实（OFI-RF）是仙人掌科的一员，原产于南美洲。对该植物的植物化学评估揭示了多种生物活性成分；因此，本研究通过评估其抗糖尿病、抗氧化和抗高胆固醇特性，探讨了正丁醇提取物（BE）和乙酸乙酯提取物（EE）的药用价值。根据既定方案，使用选定的溶剂进行植物化学提取，然后评估富含酚类和甜菜红素提取物的药理作用。结果表明，正丁醇是提取多酚类物质最有效的溶剂，其次是乙酸乙酯，产率分别为：148.91±0.95和110.96±0.61μg/g。使用超高效液相色谱/高分辨质谱/串联质谱（UPLC/HESI-MS/MS）对OFI-RF进行鉴定分析，结果显示有101种不同的代谢产物，包括多酚类物质（酚酸、酚糖苷、黄烷醇、黄烷酮醇、黄酮类化合物和双黄酮类化合物）、生物碱、吡啶、甜菜色素、香豆素、维生素、脂肪酸和其他治疗性化合物。生物学研究（体外和体内）表明，EE和BE均表现出显著的抗糖尿病、抗氧化和抗高胆固醇活性。这些发现通过组织病理学检查得到了进一步支持。
