Ziegler M L, Sisken J E
Exp Cell Res. 1985 May;158(1):263-6. doi: 10.1016/0014-4827(85)90449-5.
We have examined the effects of diamide, an oxidizer of glutathione, on the progress of HeLa cells through the cell cycle. At concentrations which do not significantly alter generation time, anaphase or cytokinesis, diamide causes a two-fold increase in the duration of metaphase. At 3 X 10(-8) M, ouabain also prolongs metaphase without effect on anaphase or cytokinesis, though with a different time course. The data suggest that the metaphase stage of mitosis is particularly sensitive to alterations in both sulfhydryl groups and Na+ levels but that the effects of diamide are probably not primarily due to the oxidation of sulfhydryl groups of the Na+/K+-ATPase.
我们研究了谷胱甘肽氧化剂二酰胺对HeLa细胞通过细胞周期进程的影响。在不显著改变细胞世代时间、后期或胞质分裂的浓度下，二酰胺会使中期持续时间增加两倍。在3×10⁻⁸ M时，哇巴因也会延长中期，对后期或胞质分裂无影响，不过时间进程不同。数据表明，有丝分裂的中期阶段对巯基和Na⁺水平的改变特别敏感，但二酰胺的作用可能并非主要由于Na⁺/K⁺-ATP酶的巯基氧化。
