Moronkeji Akinpelu, Olayanju Ayodeji, Adeniyi Temidayo Daniel, Atere Adedeji David, Moronkeji Adebimpe Iyanuoluwa, Igunbor Michael Chuks, Oyeleke Abiodun, Akinbo Frederick Olusegun
Department of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Medical Science, Ondo State, Nigeria.
Department of Biomolecular Sciences, Faculty of Health Science, Social Care and Education, Kingston University London, United Kingdom.
Environ Anal Health Toxicol. 2024 Dec;39(4):e2024030-0. doi: 10.5620/eaht.2024030. Epub 2024 Dec 26.
Petroleum-powered generators are commonly used in many developing countries as an alternative to meet utility demands. Generator exhaust emission significantly contributes to air pollution, which remains a constant threat to human health due to the presence of aromatic hydrocarbons and other harmful gases. This study assessed oxidative stress parameters in response to exhaust emission from gasoline generator engine in adult male wistar rats. Forty-eight (48) adult wistar rats weighing between 180-200g were randomly allocated to four (4) groups (A-D) of twelve (12) rats each. After the acclimatization period, the control group (A) were kept unexposed, whereas rats in groups (C-D) were exposed daily at 2, 4, and 8-hour intervals for 4, 8, and 12 weeks, respectively. Tissue samples were obtained at four weeks intervals. Fresh lung tissues weighing 1g were rinsed twice in phosphate-buffered saline (PBS, pH 8.0), homogenized and centrifuged at 3000 revolutions per minute for 20 min at 4°C. Supernatant levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), reduced glutathione (GSH), nitric oxide (NO), hydrogen peroxide (H2O2), catalase (CAT), and total antioxidant status (TAS) were determined using standard protocols. The findings revealed elevated oxidant levels of MDA, NO, and H2O2, whereas SOD, GPX, GST, GSH, CAT, and TAS were significantly reduced across the exposure time points compared to the unexposed control rats (p < 0.05). The research findings revealed that exposure to emissions from gasoline generators induced oxidative stress in the exposed rats, with the extent of disruption to their oxidative balance dependent on the duration and length of exposure time.
在许多发展中国家，石油驱动的发电机被普遍用作满足公用事业需求的替代方案。发电机废气排放是空气污染的重要来源，由于存在芳烃和其他有害气体，这对人类健康始终构成威胁。本研究评估了成年雄性Wistar大鼠对汽油发电机发动机废气排放的氧化应激参数反应。将48只体重在180 - 200克之间的成年Wistar大鼠随机分为4组（A - D），每组12只。在适应期后，对照组（A）不进行暴露处理，而C组至D组的大鼠分别以2小时、4小时和8小时的间隔每天暴露4周、8周和12周。每隔4周采集组织样本。取1克新鲜肺组织在磷酸盐缓冲盐水（PBS，pH 8.0）中冲洗两次，匀浆后在4℃下以每分钟3000转的速度离心20分钟。使用标准方案测定丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽S - 转移酶（GST）、还原型谷胱甘肽（GSH）、一氧化氮（NO）、过氧化氢（H2O2）、过氧化氢酶（CAT）和总抗氧化状态（TAS）的上清液水平。研究结果显示，与未暴露的对照大鼠相比，MDA、NO和H2O2的氧化剂水平升高，而在整个暴露时间点，SOD、GPX、GST、GSH、CAT和TAS均显著降低（p < 0.05）。研究结果表明，暴露于汽油发电机排放物会在暴露的大鼠中引发氧化应激，其氧化平衡的破坏程度取决于暴露时间的长短。
