Colarusso Analeigha, Williams Audrey, Gladfelter Amy S, Wirshing Alison C E, Lew Daniel J
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.
Department of Cell Biology, Duke University Medical Center, Durham, NC 27705.
bioRxiv. 2025 Jan 27:2025.01.25.634885. doi: 10.1101/2025.01.25.634885.
is a polyextremotolerant black yeast that exhibits impressive morphological plasticity. Consequently, it shows promise as a model system for investigating mechanisms of cell adaptation to different environments and the regulation of cell shape. Here, we build upon the current toolkit for working with and design and test 25 vectors with seven different codon-optimized fluorophores and three selection cassettes. This includes vectors that allow for dual expression of GFP and mCherry tagged proteins at the locus and vectors that enable homology-based deletion or C-terminal tagging of endogenous genes without the need for cloning. This versatile vector series for working with will enable a broad range of experiments in this emerging model system.
是一种多极端耐受性黑酵母，具有令人印象深刻的形态可塑性。因此，它有望成为研究细胞适应不同环境机制和细胞形状调控的模型系统。在这里，我们基于当前用于研究的工具包，设计并测试了25种载体，这些载体带有七种不同的密码子优化荧光团和三种选择盒。这包括允许在 位点双表达GFP和mCherry标记蛋白的载体，以及无需克隆即可实现基于同源性的内源性基因缺失或C端标记的载体。这个用于研究 的多功能载体系列将能够在这个新兴的模型系统中进行广泛的实验。
